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Chromosome spreads with centromere staining in mouse oocytes.
MedLine Citation:
PMID:  23138954     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This chapter describes a technique for performing chromosome spreads from mouse oocytes. It is based on a previously described protocol (Hodges and Hunt, Chromosoma 111: 165-169, 2002), which we have modified. Chromosomes are stained with either Propidium Iodide or Hoechst. This spreading technique allows for simultaneous immunostaining of proteins associated with chromosomes. It is very useful to stain spreads with CREST serum which labels kinetochores, to be able to distinguish bivalents (chromosome pairs), dyads or univalents (paired sister chromatids), and single sister chromatids without ambiguity.
Authors:
Jean-Philippe Chambon; Khaled Hached; Katja Wassmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  203-12     Citation Subset:  IM    
Affiliation:
CNRS UMR7622 Biologie du Développement and UPMC Paris 6, Paris, France.
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