Document Detail


Chromosome replication patterns in the hyperthermophilic euryarchaea Archaeoglobus fulgidus and Methanocaldococcus (Methanococcus) jannaschii.
MedLine Citation:
PMID:  12207709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We analysed chromosome replication patterns in the two hyperthermophilic euryarchaea Archaeoglobus fulgidus and Methanocaldococcus(Methanococcus) jannaschii by marker frequency analysis (MFA). For A. fulgidus, the central region of the chromosomal physical map displayed a higher relative abundance in gene dosage during exponential growth, with two continuous gradients to a region of lower abundance at the diametrically opposite side of the genome map. This suggests bidirectional replication of the A. fulgidus chromosome from a single origin. The organization of the putative replication origin region relative to the cdc6, mcm and DNA polymerase genes differed from that reported for Pyrococcus species. No single replication origin or termination regions could be identified for M. jannaschii, adding to the list of unusual properties of this organism. The organization of the A. fulgidus cell cycle was characterized by flow cytometry analysis of the samples from which genomic DNA was extracted for MFA. The relative lengths of the cell cycle periods were found to be similar to those of crenarchaea.
Authors:
Sophie Maisnier-Patin; Laurence Malandrin; Nils-Kåre Birkeland; Rolf Bernander
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  45     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-04     Completed Date:  2002-10-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1443-50     Citation Subset:  IM    
Affiliation:
Department of Bacteriology, Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden. Sophie.Maisnier@smi.ki.se
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MeSH Terms
Descriptor/Qualifier:
Archaeoglobus fulgidus / cytology,  genetics*,  growth & development
Base Sequence
Cell Cycle
Chromosomes, Archaeal / genetics*
DNA, Archaeal / genetics
Genetic Markers
Genome, Archaeal
Methanococcus / cytology,  genetics*,  growth & development
Physical Chromosome Mapping
Replication Origin
Chemical
Reg. No./Substance:
0/DNA, Archaeal; 0/Genetic Markers
Comments/Corrections
Erratum In:
Mol Microbiol 2002 Oct;46(2):599

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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