Document Detail

Chromosome associations in budding yeast caused by integrated tandemly repeated transgenes.
MedLine Citation:
PMID:  11884520     Owner:  NLM     Status:  MEDLINE    
The binding of GFP-tagged tetracycline repressor (TetR) molecules to chromosomally integrated tetracycline operator (tetO) sequence repeats has been used as a system to study chromosome behaviour microscopically in vivo. We found that these integrated transgenes influence the architecture of yeast interphase nuclei, as chromosomal loci with tandem repeats of exogenous tetO sequences are frequently associated. These associations occur only if TetR molecules are present. tetO tandem repeats associate regardless of their chromosomal context. When they are present at a proximal and a distal chromosomal position, they perturb the normal polarized Rabl-arrangement of chromosome arms by recruiting chromosome ends to the centromeric pole of the nucleus. Associations are established at G1 and are reduced during S-phase and mitosis. This system may serve as a model for the role of DNA sequence-specific binding proteins in imposing nonrandom distribution of chromosomes within the nucleus.
Jörg Fuchs; Alexander Lorenz; Josef Loidl
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  115     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-08     Completed Date:  2002-10-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1213-20     Citation Subset:  IM    
Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria.
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MeSH Terms
Bacterial Proteins / genetics,  metabolism
Carrier Proteins*
Cell Nucleus / chemistry,  ultrastructure
Centromere / metabolism
Chromosomes, Fungal* / metabolism,  ultrastructure
Repressor Proteins / metabolism
Saccharomycetales / drug effects,  genetics*,  growth & development,  ultrastructure
Tandem Repeat Sequences
Reg. No./Substance:
0/Bacterial Proteins; 0/Carrier Proteins; 0/Repressor Proteins; 0/Tet O resistance protein, Bacteria; 0/tetracycline resistance-encoding transposon repressor protein

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