Document Detail

Chromosome analysis of single-pronuclear haploid parthenogenetic blastocysts and their inner cell mass derivatives.
MedLine Citation:
PMID:  3655645     Owner:  NLM     Status:  MEDLINE    
Single-pronuclear haploid parthenogenetically activated mouse embryos were transferred to the oviducts of suitable recipients. One group of embryos was isolated at the morula stage and subsequently allowed to develop to the expanded blastocyst stage in vitro. Intact embryos were either analysed by the air-drying technique at that stage to determine their total cell number and ploidy, or treated by immunosurgery to isolate their inner cell mass. These were either analysed to establish their total cell number and ploidy, or retained in culture for an additional 24h or 72h. The inner cell mass derivatives were then analysed to establish the total cell number and ploidy. A second group of recipients was ovariectomized on the 4th day of pseudopregnancy, treated with Depo-Provera and blastocysts recovered 5 or 6 days later. The 'delayed' blastocysts recovered were treated by immunosurgery, and the inner cell masses isolated and either analysed at this time or transferred to culture for 72h, 96h or 144h. As in the previous groups, the inner cell mass derivatives were analysed to establish the total cell population present and their ploidy. The analysis of this material was found to be technically particularly difficult, though in general the non-'delayed' embryos and their inner cell mass derivatives yielded higher success rates than the 'delayed' inner cell mass derivatives. The 'delayed' inner cell masses initially contained on average about twice the number of cells compared to the number present in those isolated from the non-'delayed' expanded blastocysts.(ABSTRACT TRUNCATED AT 250 WORDS)
M T Schnebelen; M H Kaufman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of embryology and experimental morphology     Volume:  98     ISSN:  0022-0752     ISO Abbreviation:  J Embryol Exp Morphol     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1987-11-18     Completed Date:  1987-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7906439     Medline TA:  J Embryol Exp Morphol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  167-74     Citation Subset:  IM    
Department of Anatomy, University of Cambridge, UK.
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MeSH Terms
Blastocyst / analysis*,  cytology
Cell Aggregation
Cell Count
Cells, Cultured
Chromosomes / analysis*
Time Factors

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