Document Detail


Chromosome analysis of multipronuclear human oocytes after in vitro fertilization.
MedLine Citation:
PMID:  1766925     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multipronuclear human eggs are frequent after in vitro fertilization. Their chromosome analysis can provide useful information. Before cleavage it can confirm the suspected polyploidy. Among the cleaved multipronuclear eggs it provides an estimation of the incidence of the possible return to diploidy. Ninety-four multipronuclear eggs were fixed at the first, second, or third cleavage according to the air-drying method of Tarkowski with or without colchicine exposure: 60 were successfully analysed. Twelve were stopped before cleavage (six without colchicine treatment and six with colchicine treatment). They were polyploid, confirming the cytological observation. Forty-eight eggs cleaved and were stopped by colchicine treatment and karyotyped. Seventeen eggs (35 per cent) had produced diploid embryos. Mosaicism was frequent (15 cases, 31 per cent). Triploidy was not frequent (8 eggs, 17 per cent). Haploidy constituted the remaining cases (8 eggs, 17 per cent). Our data indicate that the initial count of pronuclei is a reliable test. Multipronuclear one-cell oocytes were confirmed to be polyploid. Furthermore, the developmental capacity of the multipronuclear oocytes is variable. Most of them cleaved. However, many multipronuclear oocytes led to diploid cleaving eggs.
Authors:
B Martin-Pont; J Selva; M Bergere; C Fillion; J N Hugues; A Tamboise; E Tamboise
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  11     ISSN:  0197-3851     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1992-02-20     Completed Date:  1992-02-20     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  501-7     Citation Subset:  IM    
Affiliation:
Laboratoire d'Histologie Embryologie Cytogénétique, CHU Bobigny, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Cleavage Stage, Ovum / ultrastructure
Female
Fertilization in Vitro*
Humans
Karyotyping
Mosaicism
Oocytes / ultrastructure
Polyploidy*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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