Document Detail


Chromosome analyses of human mammary epithelial cells at stages of chemical-induced transformation progression to immortality.
MedLine Citation:
PMID:  2702624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Benzo(a)pyrene induced extended life (EL) (i.e., a longer than normal proliferative lifespan before senescence) of human breast cells in culture. From many EL cell cultures immortalized cells emerged only once in each of two separate experiments. The original EL cells were mostly normal diploid with only a small percentage of tetraploid cells. The two immortalized cell lines, however, were near diploid, each containing a set of chromosomal aberrations that were present in all the cells analyzed, confirming the clonal origin of both cell lines. For cell line 184A1 the aberrations consisted of deficiencies only, whereas a combination of deficiencies and duplications characterized the 184B5 line. None of the individual aberrations of each set were shared by both cell lines. Both sets of aberrations have remained stable for over 150 population doublings, while some of the other chromosomes showed breakage and reunions. These data are discussed in regard to types of mutations in the sequence of changes from primary to immortalized cells, and it is concluded that the sets of aberrations most likely originated as multiple events in a single cell.
Authors:
K H Walen; M R Stampfer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  37     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-05-22     Completed Date:  1989-05-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  249-61     Citation Subset:  IM    
Affiliation:
Viral and Rickettsial Disease Laboratory, California State Department of Health Services, Berkeley 94704.
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MeSH Terms
Descriptor/Qualifier:
Adult
Benzo(a)pyrene
Breast / drug effects,  pathology,  ultrastructure*
Cell Line
Cell Transformation, Neoplastic*
Chromosome Aberrations*
Epithelium / ultrastructure
Female
Humans
Grant Support
ID/Acronym/Agency:
CA-24844/CA/NCI NIH HHS; CA-30028/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
50-32-8/Benzo(a)pyrene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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