Document Detail

Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.
MedLine Citation:
PMID:  21124819     Owner:  NLM     Status:  MEDLINE    
The Caenorhabditis elegans inner nuclear envelope protein matefin/SUN-1 plays a conserved, pivotal role in the process of genome haploidization. CHK-2-dependent phosphorylation of SUN-1 regulates homologous chromosome pairing and interhomolog recombination in Caenorhabditis elegans. Using time-lapse microscopy, we characterized the movement of matefin/SUN-1::GFP aggregates (the equivalent of chromosomal attachment plaques) and showed that the dynamics of matefin/SUN-1 aggregates remained unchanged throughout leptonene/zygotene, despite the progression of pairing. Movement of SUN-1 aggregates correlated with chromatin polarization. We also analyzed the requirements for the formation of movement-competent matefin/SUN-1 aggregates in the context of chromosome structure and found that chromosome axes were required to produce wild-type numbers of attachment plaques. Abrogation of synapsis led to a deceleration of SUN-1 aggregate movement. Analysis of matefin/SUN-1 in a double-strand break deficient mutant revealed that repair intermediates influenced matefin/SUN-1 aggregate dynamics. Investigation of movement in meiotic regulator mutants substantiated that proper orchestration of the meiotic program and effective repair of DNA double-strand breaks were necessary for the wild-type behavior of matefin/SUN-1 aggregates.
Antoine Baudrimont; Alexandra Penkner; Alexander Woglar; Thomas Machacek; Christina Wegrostek; Jiradet Gloggnitzer; Alexandra Fridkin; Franz Klein; Yosef Gruenbaum; Pawel Pasierbek; Verena Jantsch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-24
Journal Detail:
Title:  PLoS genetics     Volume:  6     ISSN:  1553-7404     ISO Abbreviation:  PLoS Genet.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-12-02     Completed Date:  2011-02-28     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101239074     Medline TA:  PLoS Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1001219     Citation Subset:  IM    
Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
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MeSH Terms
Caenorhabditis elegans / cytology*,  metabolism*
Caenorhabditis elegans Proteins / chemistry,  metabolism*
Cell Cycle Proteins / metabolism*
Cell Nucleus / metabolism
Chromatin / metabolism
Chromosomes / metabolism*
Cytoskeleton / metabolism
DNA Breaks, Double-Stranded
Meiotic Prophase I*
Models, Biological
Nuclear Proteins / metabolism*
Protein Structure, Quaternary
Protein Transport
Receptors, Cytoplasmic and Nuclear / chemistry,  metabolism*
Synaptonemal Complex / metabolism
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Cell Cycle Proteins; 0/Chromatin; 0/KDP-1 protein, C elegans; 0/Nuclear Proteins; 0/Receptors, Cytoplasmic and Nuclear; 0/matefin protein, C elegans

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