| Chromosome 6 encoded RNaseT2 protein is a cell growth regulator. | |
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MedLine Citation:
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PMID: 19382914 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown by chromosome transfer technique that chromosome 6 alters the phenotype of a variety of tumour cells and SV40 immortalized cells. We present here the phenotypic effects of the ectopic expression of RNaseT2, a highly conserved ribonuclease encoded by chromosome 6q27, in SV40 immortalized cell lines. We contrast our findings with those reported for ovarian carcinoma cell lines and an SV40 immortalized cell line transfected with RNaseT2. Although RNaseT2 expression is elevated in normal diploid fibroblasts approaching senescence (passage 64), forced expression of the gene in immortalized cells does not cause them to senesce. A significant reduction was observed in colony forming efficiency, anchorage independence and growth rate of cells transfected with RNaseT2. The levels of transcripts involved in Akt signalling pathway, cell cycle control and pathways related to cell proliferation decreased 2-10-folds in SV40 immortalized cells in response to RNaseT2 expression. Interestingly, some immortalized cells expressed alternatively spliced transcript variants instead of the full-length RNaseT2 transcript. Our results are consistent with the conclusion that RNaseT2 is a cell growth regulator and it does not induce senescence in SV40 immortalized cell lines. |
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Authors:
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Jinglan Liu; Vikramjit K Zhawar; Gurpreet Kaur; G Pal Kaur; Jon Kimball Deriel; Raj P Kandpal; Raghbir S Athwal |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-03-27 |
Journal Detail:
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Title: Journal of cellular and molecular medicine Volume: 14 ISSN: 1582-4934 ISO Abbreviation: J. Cell. Mol. Med. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-07-21 Completed Date: 2010-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101083777 Medline TA: J Cell Mol Med Country: England |
Other Details:
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Languages: eng Pagination: 1146-55 Citation Subset: IM |
Affiliation:
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Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing
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genetics Cell Adhesion Cell Line, Transformed Cell Proliferation Cell Transformation, Viral Chromosomes, Human, Pair 6 / enzymology* Colony-Forming Units Assay Gene Expression Profiling Gene Expression Regulation, Enzymologic Humans Proto-Oncogene Proteins c-akt / metabolism RNA, Messenger / genetics, metabolism Ribonucleases / genetics, metabolism* Signal Transduction Simian virus 40 / physiology Transfection Tumor Suppressor Proteins / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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CA74983/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Tumor Suppressor Proteins; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 3.1.-/Ribonucleases; EC 3.1.27.-/RNASET2 protein, human |
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