Document Detail


Chromosome 14 marker appearance in a human B lymphoblastoid cell line of nonmalignant origin.
MedLine Citation:
PMID:  3080224     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human B cell lymphoblastoid cell line of nonmalignant origin, derived 16 years ago, now carries a 14q+ marker chromosome in 100% of the cells analyzed. This marker was not apparent in karyotypes prepared 1 year after establishment of the line and, thus, presumably arose in culture. The cell line now grows more rapidly than typical B cell cultures and has an unusually high cloning efficiency (60%-90%). Using Southern blot hybridization, we have analyzed the arrangement of 13 onc genes that are often associated with human malignancy, including c-myc. None of these onc genes appear to be rearranged in this cell line.
Authors:
D W Yandell; J B Little
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  20     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-03-13     Completed Date:  1986-03-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  231-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
B-Lymphocytes / pathology*
Cell Line
Chromosomes, Human, 13-15*
Humans
Immunoglobulin Heavy Chains / genetics
Karyotyping
Oncogenes
Trisomy
Grant Support
ID/Acronym/Agency:
CA-09078/CA/NCI NIH HHS; CA-11751/CA/NCI NIH HHS; ES-00002/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Immunoglobulin Heavy Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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