Document Detail

Chromosomal variation in neurons of the developing and adult mammalian nervous system.
MedLine Citation:
PMID:  11698687     Owner:  NLM     Status:  MEDLINE    
A basic assumption about the normal nervous system is that its neurons possess identical genomes. Here we present direct evidence for genomic variability, manifested as chromosomal aneuploidy, among developing and mature neurons. Analysis of mouse embryonic cerebral cortical neuroblasts in situ detected lagging chromosomes during mitosis, suggesting the normal generation of aneuploidy in these somatic cells. Spectral karyotype analysis identified approximately 33% of neuroblasts as aneuploid. Most cells lacked one chromosome, whereas others showed hyperploidy, monosomy, and/or trisomy. The prevalence of aneuploidy was reduced by culturing cortical explants in medium containing fibroblast growth factor 2. Interphase fluorescence in situ hybridization on embryonic cortical cells supported the rate of aneuploidy observed by spectral karyotyping and detected aneuploidy in adult neurons. Our results demonstrate that genomes of developing and adult neurons can be different at the level of whole chromosomes.
S K Rehen; M J McConnell; D Kaushal; M A Kingsbury; A H Yang; J Chun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  98     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-07     Completed Date:  2001-12-07     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13361-6     Citation Subset:  IM    
Department of Pharmacology, School of Medicine, University of California, San Diego, CA 92093-0636, USA.
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MeSH Terms
Cerebral Cortex / ultrastructure*
Flow Cytometry
Genetic Variation*
In Situ Hybridization, Fluorescence
Mice, Inbred BALB C
Neurons / ultrastructure*

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