Document Detail


Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells.
MedLine Citation:
PMID:  9724779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse has become an increasingly important organism for modeling human diseases and for determining gene function in a mammalian context. Unfortunately, transposon-tagged mutagenesis, one of the most valuable tools for functional genomics, still is not available in this organism. On the other hand, it has long been speculated that members of the Tc1/mariner-like elements may be less dependent on host factors and, hence, can be introduced into heterologous organisms. However, this prediction has not been realized in mice. We report here the chromosomal transposition of the Sleeping Beauty (SB) element in mouse embryonic stem cells, providing evidence that it can be used as an in vivo mutagen in mice.
Authors:
G Luo; Z Ivics; Z Izsvák; A Bradley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  95     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-09-28     Completed Date:  1998-09-28     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10769-73     Citation Subset:  IM    
Affiliation:
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Line
Chromosome Mapping*
DNA Primers
DNA Transposable Elements*
DNA-Binding Proteins
Embryo, Mammalian / cytology,  metabolism*
Humans
Mice
Mutagenesis
Stem Cells / metabolism*
Transposases / metabolism*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA Transposable Elements; 0/DNA-Binding Proteins; 0/mariner transposases; EC 2.7.7.-/Transposases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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