Document Detail

Chromosomal response of human lymphocytes to streptozotocin.
MedLine Citation:
PMID:  12052504     Owner:  NLM     Status:  MEDLINE    
The induction of chromosomal aberrations (CAs) and sister-chromatid exchanges (SCEs) by the methylating agent streptozotocin (STZ) and the effect of this compound on mitotic index (MI) and cell cycle progression in human lymphocytes were investigated. Unstimulated (G(0)) or cycling lymphocytes derived from whole blood or purified lymphocyte cultures were pulse-treated with increasing doses of STZ for 0.5-24h. Induction of CAs by STZ was only observed in cycling lymphocytes derived from whole blood cultures (WBC) (P<0.05). On the contrary, STZ produced a significant and dose-response increase in the yield of SCEs in unstimulated as well as cycling lymphocytes (P<0.05). In addition, STZ induced a dose-dependent decrease in the MI but had a slight effect on cell cycle progression. These results suggest that SCEs are the most sensitive endpoint for evaluating the chromosomal effects of STZ on these cells.
Alejandro D Bolzán; Martha S Bianchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  503     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-07     Completed Date:  2002-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  63-8     Citation Subset:  IM    
Laboratorio de Citogenética y Mutagénesis, Instituto Multidisciplinario de Biología Celular (IMBICE), C.C. 403, 1900 La Plata, Argentina.
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MeSH Terms
Cell Cycle / drug effects
Chromosome Aberrations / chemically induced*
Dose-Response Relationship, Drug
Lymphocytes / drug effects*,  ultrastructure
Mitotic Index
Sister Chromatid Exchange / drug effects*
Streptozocin / toxicity*
Reg. No./Substance:

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