| Chromosomal replication asynchrony of a human breast carcinoma cell line. I. studied by continuous BrdU incorporation and G-banding analysis. | |
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MedLine Citation:
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PMID: 7172164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Continuous BrdU incorporation and the Giemsa staining technique were used to study the cell cycle kinetics of a human breast tumor cell line. It was found that the interchromosomal replication pattern of the neoplastic cell was significantly different from that of normal cells in two respects. First, the pattern is highly asynchronous; within a single cell there are chromosomes at different replication cycles; that is, some chromosomes complete their DNA replication before others begin. Second, the replication schedule for the chromosomes, as identified by superimposing the BrdU-Giemsa technique on the trypsin G-banding technique, is relatively consistent within the cell line but differs from that of normal cells. Some chromosomes that replicate late in normal human lymphocytes and fibroblasts replicate early in this cell line. In contrast to the unusual interchromosomal replication pattern, gross analysis of the intrachromosomal replication schedule shows no apparent difference from that reported for normal cells. The asynchrony phenomenon reported here may be associated with the etiology of aneuploidy in neoplasia. |
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Authors:
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N Wang; B Trend; H L Kaung; T Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer genetics and cytogenetics Volume: 7 ISSN: 0165-4608 ISO Abbreviation: Cancer Genet. Cytogenet. Publication Date: 1982 Oct |
Date Detail:
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Created Date: 1983-02-14 Completed Date: 1983-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7909240 Medline TA: Cancer Genet Cytogenet Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 173-80 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Azure Stains
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diagnostic use Breast Neoplasms / genetics*, pathology Bromodeoxyuridine / diagnostic use Cell Cycle* Cell Line Chromosome Banding Chromosome Mapping Chromosomes / ultrastructure DNA Replication* Female Humans Karyotyping |
| Chemical | |
Reg. No./Substance:
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0/Azure Stains; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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