Document Detail

Chromosomal microarray analysis as a first-line test in pregnancies with a priori low risk for the detection of submicroscopic chromosomal abnormalities.
MedLine Citation:
PMID:  23211699     Owner:  NLM     Status:  MEDLINE    
In this study, we aimed to explore the utility of chromosomal microarray analysis (CMA) in groups of pregnancies with a priori low risk for detection of submicroscopic chromosome abnormalities, usually not considered an indication for testing, in order to assess whether CMA improves the detection rate of prenatal chromosomal aberrations. A total of 3000 prenatal samples were processed in parallel using both whole-genome CMA and conventional karyotyping. The indications for prenatal testing included: advanced maternal age, maternal serum screening test abnormality, abnormal ultrasound findings, known abnormal fetal karyotype, parental anxiety, family history of a genetic condition and cell culture failure. The use of CMA resulted in an increased detection rate regardless of the indication for analysis. This was evident in high risk groups (abnormal ultrasound findings and abnormal fetal karyotype), in which the percentage of detection was 5.8% (7/120), and also in low risk groups, such as advanced maternal age (6/1118, 0.5%), and parental anxiety (11/1674, 0.7%). A total of 24 (0.8%) fetal conditions would have remained undiagnosed if only a standard karyotype had been performed. Importantly, 17 (0.6%) of such findings would have otherwise been overlooked if CMA was offered only to high risk pregnancies.The results of this study suggest that more widespread CMA testing of fetuses would result in a higher detection of clinically relevant chromosome abnormalities, even in low risk pregnancies. Our findings provide substantial evidence for the introduction of CMA as a first-line diagnostic test for all pregnant women undergoing invasive prenatal testing, regardless of risk factors.
Francesco Fiorentino; Stefania Napoletano; Fiorina Caiazzo; Mariateresa Sessa; Sara Bono; Letizia Spizzichino; Anthony Gordon; Andrea Nuccitelli; Giuseppe Rizzo; Marina Baldi
Related Documents :
58449 - An in vivo giemsa chromosome banding technique.
15829739 - Use reference bands to accurately estimate iscn band levels 400, 550, and 850.
7593159 - A three-dimensional structural dissection of drosophila polytene chromosomes.
3059479 - Sem of canine chromosomes: normal structure and the effects of whole-body irradiation.
24521639 - Extracellular chromosome 21-derived micrornas in euploid & aneuploid pregnancies.
18470229 - Zebrafish chromosome banding.
15211659 - A new patient with pure trisomy 4p resulting from isochromosome formation and whole arm...
1396599 - Comparison of the expression of the seven ribosomal rna operons in escherichia coli.
20302859 - Remodeling of nuclear architecture by the thiodioxoxpiperazine metabolite chaetocin.
Publication Detail:
Type:  Journal Article     Date:  2012-12-05
Journal Detail:
Title:  European journal of human genetics : EJHG     Volume:  21     ISSN:  1476-5438     ISO Abbreviation:  Eur. J. Hum. Genet.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-13     Completed Date:  2013-11-04     Revised Date:  2014-07-01    
Medline Journal Info:
Nlm Unique ID:  9302235     Medline TA:  Eur J Hum Genet     Country:  England    
Other Details:
Languages:  eng     Pagination:  725-30     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Abnormal Karyotype
Chromosome Aberrations*
Chromosome Disorders / diagnosis*,  etiology,  genetics*
Genetic Testing
Maternal Age
Microarray Analysis / methods*
Prenatal Diagnosis
Ultrasonography, Prenatal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Deep-intronic ATM mutation detected by genomic resequencing and corrected in vitro by antisense morp...
Next Document:  Low prevalence of SLX4 loss-of-function mutations in non-BRCA1/2 breast and/or ovarian cancer famili...