Document Detail


Chromosomal localization of zebrafish AluI repeats by primed in situ (PRINS) labeling.
MedLine Citation:
PMID:  10343094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two zebrafish AluI repeats were localized in metaphase chromosomes by means of the primed in situ (PRINS) labeling technique, using oligonucleotide primers based on published sequences. An AT-rich, tandemly repeated, long AluI restriction fragment (RFAL1) labeled the (peri)centromeric regions of all chromosomes. The GC-rich short fragment (RFAS) was found to be localized in the paracentromeric regions of 17 chromosome pairs, which were mostly subtelocentric. The RFAS labeling pattern generally fits the previously described chromomycin A3 (CMA3) staining pattern. The differential composition of heterochromatin in zebrafish chromosomes is discussed.
Authors:
L Sola; I Gabrielli; S De Innocentiis; E Gornung
Related Documents :
2591184 - Visual and tactile observational error: comparative probing reliability with recession ...
10659794 - Distribution of tgg repeat-related sequences in 'trovita' orange (citrus sinensis osbec...
8872464 - Founder effect in spinal and bulbar muscular atrophy (sbma).
9804814 - New 5'-(cgg)n-3' repeats in the human genome.
22797674 - Cloning and characterization of chromosomal markers from a cot-1 library of peanut ( ar...
20439644 - Cytogenetics of childhood acute myeloid leukemia: united kingdom medical research counc...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  84     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1999  
Date Detail:
Created Date:  1999-06-25     Completed Date:  1999-06-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  28-30     Citation Subset:  IM    
Affiliation:
Department of Animal and Human Biology, University of Rome 1, Rome, Italy. sola@uniroma.it
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alu Elements*
Animals
Base Sequence
Centromere / genetics
Chromosome Mapping / methods*
DNA Primers / genetics
Female
Heterochromatin / genetics
In Situ Hybridization, Fluorescence / methods*
Male
Zebrafish / genetics*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Heterochromatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Integrated genetic and physical map of the 1q31-->q32.1 region, encompassing the RP12 locus, the F13...
Next Document:  Molecular cloning of a SALL1-related pseudogene and mapping to chromosome Xp11.2.