Document Detail

Chromosomal localization of zebrafish AluI repeats by primed in situ (PRINS) labeling.
MedLine Citation:
PMID:  10343094     Owner:  NLM     Status:  MEDLINE    
Two zebrafish AluI repeats were localized in metaphase chromosomes by means of the primed in situ (PRINS) labeling technique, using oligonucleotide primers based on published sequences. An AT-rich, tandemly repeated, long AluI restriction fragment (RFAL1) labeled the (peri)centromeric regions of all chromosomes. The GC-rich short fragment (RFAS) was found to be localized in the paracentromeric regions of 17 chromosome pairs, which were mostly subtelocentric. The RFAS labeling pattern generally fits the previously described chromomycin A3 (CMA3) staining pattern. The differential composition of heterochromatin in zebrafish chromosomes is discussed.
L Sola; I Gabrielli; S De Innocentiis; E Gornung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  84     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1999  
Date Detail:
Created Date:  1999-06-25     Completed Date:  1999-06-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  28-30     Citation Subset:  IM    
Department of Animal and Human Biology, University of Rome 1, Rome, Italy.
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MeSH Terms
Alu Elements*
Base Sequence
Centromere / genetics
Chromosome Mapping / methods*
DNA Primers / genetics
Heterochromatin / genetics
In Situ Hybridization, Fluorescence / methods*
Zebrafish / genetics*
Reg. No./Substance:
0/DNA Primers; 0/Heterochromatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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