Document Detail

Chromosomal localization and distribution of simple sequence repeats and the Arabidopsis-type telomere sequence in the genome of Cicer arietinum L.
MedLine Citation:
PMID:  9543012     Owner:  NLM     Status:  MEDLINE    
We used fluorescence in situ hybridization to probe the physical organization of five simple sequence repeat motifs and the Arabidopsis-type telomeric repeat in metaphase chromosomes and interphase nuclei of chickpea (Cicer arietinum L.). Hybridization signals were observed with the whole set of probes and on all chromosomes, but the distribution and intensity of signals varied depending on the motif. On root-tip metaphase chromosomes, CA and GATA repeats were mainly restricted to centromeric areas, with additional GATA signals along some chromosomes. TA, A and AAC repeats were organized in a more dispersed manner, with centromeric regions being largely excluded. In interphase nuclei of the inner integument, CA and GATA signals predominantly occurred in the heterochromatic endochromocentres, whereas the other motifs were found both in eu- and heterochromatin. The distribution of the Arabidopsis-type telomeric repeat (TTTAGGG)n on metaphase chromosomes was found to be quite exceptional. One major cluster of repeats was spread along the short arm of chromosome B, whereas a second, weaker signal occurred interstitially on chromosome A. Only faint and inconsistent hybridization signals were visualized with the same probe at the chromosomal termini.
G Gortner; M Nenno; K Weising; D Zink; W Nagl; G Kahl
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  6     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-05-14     Completed Date:  1998-05-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  97-104     Citation Subset:  IM    
Plant Molecular Biology, Biocentre, University of Frankfurt, Frankfurt/Main, Germany.
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MeSH Terms
Arabidopsis / genetics
Chromosome Mapping
Chromosomes / genetics*
Dinucleotide Repeats / genetics
Fabaceae / genetics*
In Situ Hybridization, Fluorescence / methods*
Oligonucleotide Probes
Plants, Medicinal*
Repetitive Sequences, Nucleic Acid / genetics*
Telomere / genetics*
Reg. No./Substance:
0/Oligonucleotide Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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