Document Detail


Chromosomal instability and telomere lengths of each chromosomal arm measured by Q-FISH in human fibroblast strains prior to replicative senescence.
MedLine Citation:
PMID:  20817007     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We monitored the telomere lengths and chromosomal instability characteristics of fibroblasts at different population doubling levels (PDLs) to gain further insight into the role of telomere shortening in chromosomal instability. We used 7 normal diploid human fibroblast strains (TIG-1, 3, 7, 103, 104, 112, and 114) and a quantitative fluorescence in situ hybridization method to measure telomere lengths of the p- and q-arms of individual chromosomes. We also enumerated morphologic signs of chromosomal instability, including fusion or loss of chromosomes, and anaphase bridges. In strains TIG-1, 3, 7, 103, and 114 at the late (phase 3) stage (≧40PDLs), 29 (96.6%) of 30 fusions were associated with one or both of the chromosomal arms that bear significantly shorter telomeres in those populations. In TIG-1 at 62PDL, 6 fusions were associated with Xq (n=3), 21q (n=3), and other (n=6) chromosomes. Xq and 21q had significantly shorter telomeres, and anaphase bridges were often associated with chromosomes X and/or 21 (74.6%). Our results indicate that chromosomes having excessively shortened telomeres at late PDLs begin to show features of instability such as fusions and anaphase bridges.
Authors:
Kaiyo Takubo; Junko Aida; Naotaka Izumiyama; Naoshi Ishikawa; Mutsunori Fujiwara; Steven S S Poon; Hiroshi Kondo; Makoto Kammori; Masaaki Matsuura; Motoji Sawabe; Tomio Arai; Duncan M Baird; Ken-Ichi Nakamura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-09
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  131     ISSN:  1872-6216     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  614-24     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, Japan. takubo@tmig.or.jp
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Anaphase / physiology
Cell Aging / physiology*
Cell Line
Chromosomal Instability*
Chromosomes, Human, Pair 21 / genetics,  metabolism*
Chromosomes, Human, X / genetics,  metabolism*
Female
Humans
In Situ Hybridization, Fluorescence
Male
Telomere / genetics,  metabolism*

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