Document Detail

Chromosomal clustering and GATA transcriptional regulation of intestine-expressed genes in C. elegans.
MedLine Citation:
PMID:  16354718     Owner:  NLM     Status:  MEDLINE    
We used mRNA tagging to identify genes expressed in the intestine of C. elegans. Animals expressing an epitope-tagged protein that binds the poly-A tail of mRNAs (FLAG::PAB-1) from an intestine-specific promoter (ges-1) were used to immunoprecipitate FLAG::PAB-1/mRNA complexes from the intestine. A total of 1938 intestine-expressed genes (P<0.001) were identified using DNA microarrays. First, we compared the intestine-expressed genes with those expressed in the muscle and germline, and identified 510 genes enriched in all three tissues and 624 intestine-, 230 muscle- and 1135 germ line-enriched genes. Second, we showed that the 1938 intestine-expressed genes were physically clustered on the chromosomes, suggesting that the order of genes in the genome is influenced by the effect of chromatin domains on gene expression. Furthermore, the commonly expressed genes showed more chromosomal clustering than the tissue-enriched genes, suggesting that chromatin domains may influence housekeeping genes more than tissue-specific genes. Third, in order to gain further insight into the regulation of intestinal gene expression, we searched for regulatory motifs. This analysis found that the promoters of the intestine genes were enriched for the GATA transcription factor consensus binding sequence. We experimentally verified these results by showing that the GATA motif is required in cis and that GATA transcription factors are required in trans for expression of these intestinal genes.
Florencia Pauli; Yueyi Liu; Yoona A Kim; Pei-Jiun Chen; Stuart K Kim
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2005-12-14
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  133     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-21     Completed Date:  2006-03-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  287-95     Citation Subset:  IM    
Department of Genetics, Stanford University, Stanford, CA 94305, USA.
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MeSH Terms
Animals, Genetically Modified
Caenorhabditis elegans / genetics*,  growth & development,  metabolism
Caenorhabditis elegans Proteins / genetics*,  metabolism
Chromosomes / genetics
GATA Transcription Factors / genetics*,  metabolism
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genes, Helminth*
Germ Cells / metabolism
Intestines / metabolism
Multigene Family
Muscles / metabolism
Mutagenesis, Site-Directed
Oligonucleotide Array Sequence Analysis
RNA, Messenger / genetics
Grant Support
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/GATA Transcription Factors; 0/RNA, Messenger

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