Document Detail


Chromosomal changes observed in immortalized human keratinocytes transformed by ionizing radiation.
MedLine Citation:
PMID:  9078297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human epithelial cancer cells were induced by concerted action of DNA tumor virus and X-ray radiation. Treatment of nontumorigenic early passage AD12-SV40 immortalized epithelial cells (RHEK-1) at passage 23 with radiation, resulted in further changes in their growth properties. One day old cultures of these RHEK-1 cells were irradiated with graded doses of X-rays (0, 2, 4, 6, and 8 Gy i.e. RHEK-1, RHEK-1/200R, RHEK-1/400R, RHEK-1/600R, and RHEK-1/800R). Morphologic alterations, the ability to grow in soft agar, and to form rapidly-growing squamous cell carcinomas in nude mice were concomitantly acquired properties of the radiation transformed cell lines RHEK-1/200R and RHEK-1/ 400R. On the basis of commonality in having addition of some extra material in chromosome 11 in the region between q14/q22 in all tumorigenic cell lines RHEK-1/200R and RHEK-1/400R, and deletion of the same region in nontumorigenic irradiated cell lines-RHEK-1/600R and RHEK-1/800R, it is deduced this region may have some important oncogene/s or other gene/s that play an important role in tumorigenesis. When compared to squamous cell carcinoma data, the duplication observed in the present study is also observed in 28 to 38% of head and neck and also in 25% of cases of untreated malignant lesions of oral squamous cell carcinoma. Thus, this study shows the correlation between in vitro induced squamous cell carcinoma to in vivo tumors.
Authors:
B Hukku; P Thraves; A Dritschilo; J S Rhim
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  93     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-04-10     Completed Date:  1997-04-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  125-39     Citation Subset:  IM    
Affiliation:
Children's Hospital of Michigan, Department of Pediatrics, Detroit 48201, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Squamous Cell / genetics*
Cell Line, Transformed
Chromosome Aberrations / genetics*
Chromosomes, Human, Pair 11 / genetics
Chromosomes, Human, Pair 3 / genetics
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Keratinocytes / pathology,  radiation effects*
Mice
Mice, Nude
Translocation, Genetic
Grant Support
ID/Acronym/Agency:
CA52945/CA/NCI NIH HHS; CA52954/CA/NCI NIH HHS; N0I-CP33063/CP/NCI NIH HHS

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