Document Detail


Chromosomal assignment of human YAC clones by fluorescence in situ hybridization: use of single-yeast-colony PCR and multiple labeling.
MedLine Citation:
PMID:  1427825     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alu-PCR provides a convenient tool for amplification of human-specific sequences from yeast DNA containing yeast artificial chromosomes (YAC) clones. PCR products can be labeled nonisotopically and hybridized in situ, and the chromosomal origin of the clones can be determined. This avoids time-consuming gel purification of the yeast artificial chromosome and the low-efficiency procedure of labeling whole yeast DNA containing the YAC. The application of Alu-PCR to single-yeast colonies permits the mapping of YACs at a very early stage of their characterization. In situ hybridization can detect clones with noncontiguous fragments of DNA, and these can be discarded without further time-consuming characterization. To increase further the potential of the method, we show the application of multicolor hybridization techniques.
Authors:
A Baldini; M Ross; D Nizetic; R Vatcheva; E A Lindsay; H Lehrach; M Siniscalco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  14     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-12-22     Completed Date:  1992-12-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  181-4     Citation Subset:  IM    
Affiliation:
Istituto di Genetica Molecolare del CNR, Porto Conte Research and Training Laboratories, Alghero, Italy.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosome Mapping* / methods*
Chromosomes, Fungal*
Fluorescence
Gene Library
Genome, Human*
Humans
In Situ Hybridization*
Molecular Sequence Data
Polymerase Chain Reaction*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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