Document Detail


Chromosomal DNA content of sweet pepper determined by association of cytogenetic and cytometric tools.
MedLine Citation:
PMID:  18347800     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires a great quantity of intact metaphasic chromosomes and methods that allow the characterization of individual chromosomes; however, the obtainment of adequate number of metaphases can be difficult in some species like C. annuum. In order to estimate the DNA content of each C. annuum var. annuum cv. "New Mexican" chromosome, flow and image cytometries were associated with the cytogenetic methodology. First, the DNA amount (2C = 6.90 pg) was established by flow cytometry. Integrated optical density (IOD) values were calculated by image cytometry for each Feulgen stained metaphasic chromosome. Then, by distributing the correspondent metaphasic value (4C = 13.80 pg) proportionally to average IOD values, the following chromosomal DNA contents were obtained in pg: 0.74 (chromosome 1), 0.67 (2), 0.61 (3, 4), 0.60 (5), 0.59 (6, 7), 0.58 (8), 0.57 (9), 0.56 (10) and 0.39 (11, 12). This study reports an alternative and reproducible technique that makes quantifying the chromosomal DNA content possible.
Authors:
Isabella Santiago de Abreu; Carlos Roberto Carvalho; Wellington Ronildo Clarindo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-18
Journal Detail:
Title:  Plant cell reports     Volume:  27     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-05     Completed Date:  2008-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1227-33     Citation Subset:  IM    
Affiliation:
Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, Viçosa, Minas Gerais CEP 36570-000, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Capsicum / genetics*
Cell Nucleus / metabolism
Chromosomes, Plant / genetics*
Cytogenetics / methods*
DNA, Plant / analysis*,  genetics
Flow Cytometry / methods*
G0 Phase
G1 Phase
Chemical
Reg. No./Substance:
0/DNA, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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