| Chromophore Exchange in the Blue Light-Sensitive Photoreceptor YtvA from Bacillus subtilis. | |
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MedLine Citation:
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PMID: 21259411 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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YtvA from Bacillus subtilis was found as the first prokaryotic phototropin-like blue-light-responsive photoreceptor. It is composed of two domains, the photoactive LOV (light, oxygen, voltage) domain, which binds a flavin mononucleotide (FMN) as a chromophore and a STAS (sulfate transporter/anti-sigma-factor antagonist) domain, which generates a physiological signal. Here we present a routine chromophore-exchange protocol that allows chemically synthesized, structurally modified chromophores instead of the naturally present flavin mononucleotide (FMN) chromophore to be introduced. FMN was exchanged for riboflavin (RF), flavin adenine dinucleotide (FAD), 7,8-didemethyl flavin mononucleotide (DMFMN), and 8-isopropyl flavin mononucleotide (iprFMN). LOV domains reconstituted with new flavins undergo the same photocycle as native YtvA LOV, consisting of triplet formation and covalent binding of the chromophore followed by a thermal recovery of the parent state, albeit with different kinetics and photophysical properties. Interestingly, the iprFMN chromophore, inducing steric hindrances to the protein, exhibits a very fast light-to-dark-conversion and shows a high fluorescence quantum yield (0.4). Incorporation of FAD causes an increase of its fluorescence quantum yield from 0.04 (H(2) O) to 0.2. |
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Authors:
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Madina Mansurova; Pierre Scheercousse; Julian Simon; Marianne Kluth; Wolfgang Gärtner |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-21 |
Journal Detail:
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Title: Chembiochem : a European journal of chemical biology Volume: - ISSN: 1439-7633 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100937360 Medline TA: Chembiochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Max Planck Institute for Bioinorganic Chemistry, Postfach 10135, 45410 Mülheim an der Ruhr (Germany), Fax: (+49) 208-306-3951. mansurova@mpi-muelheim.mpg.de. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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