Document Detail

Chromogenic derivatives of AHTN (6-acetyl-1,1,2,4,4,7-hexamethyltetralin) react with amino acids and protein in vitro. Spectral characteristics of the colour products.
MedLine Citation:
PMID:  15046824     Owner:  NLM     Status:  MEDLINE    
Three chromogenic products of AHTN have been detected after photo-oxidation and shown to react with glycine or albumin, giving rise first to a blue color, followed by pink and, finally, a dark green colour. The absorption spectrum associated with the late green colour showed an increased absorbance in the long wavelength region of the spectrum, similar to the green colour extracted from the liver of AHTN-treated animals. The colour produced by the chromogenic derivatives of AHTN and by o-diacetylbenzene (a model chromogenic compound) were tightly bound to the protein pellet and resistant to acidic pH, unlike the colour from the ninhydrin reaction. The dark green colour produced in the liver by feeding AHTN to rats was also tightly bound to proteins and stable to acidic pH. These results suggest that both the photo-oxidized chromogenic derivatives of AHTN and o-diacetylbenzene, produce coloured derivatives with amino acids and proteins by a mechanism unrelated to ninhydrin. They also suggest that a chromogenic derivative of AHTN, produced in vivo during prolonged treatment with high doses of AHTN, may be responsible for the green colour of the livers and other tissues from treated animals. The data suggest that the AHTN-derived chromogenic material is metabolite-related rather than representative of a toxic process, although further work is necessary to confirm this hypothesis.
Sandra Pipino; Anne Marie Api; Nicoletta Pons; Robert L Smith; Francesco De Matteis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  42     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-03-29     Completed Date:  2004-05-17     Revised Date:  2008-03-07    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  785-90     Citation Subset:  IM    
Department of Anatomy, Pharmacology and Forensic Medicine, Medical Pharmacology Section, Via Pietro Giuria 13, 10125 Torino, Italy.
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MeSH Terms
Amino Acids / metabolism*
Chromatography, High Pressure Liquid / methods
Chromatography, Thin Layer
Chromogenic Compounds / chemistry,  metabolism*
Hydrogen-Ion Concentration
Liver / metabolism*
Microsomes, Liver / metabolism
NADP / metabolism,  pharmacology
Naphthalenes / metabolism*
Ninhydrin / chemistry
Proteins / metabolism*
Rats, Wistar
Spectrophotometry, Ultraviolet / methods
Reg. No./Substance:
0/Amino Acids; 0/Chromogenic Compounds; 0/Naphthalenes; 0/Proteins; 0/Tetrahydronaphthalenes; 21145-77-7/acetyl methyl tetramethyl tetralin; 485-47-2/Ninhydrin; 53-59-8/NADP

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