Document Detail

Chromatographic studies of changes in binding of sulfonylurea drugs to human serum albumin due to glycation and fatty acids.
MedLine Citation:
PMID:  20974553     Owner:  NLM     Status:  MEDLINE    
This report examines the use of high-performance affinity chromatography as a screening tool for studying the change in binding by sulfonylurea drugs to the protein human serum albumin (HSA) during diabetes. The effects of both the non-enzymatic glycation of HSA and the presence of fatty acids on these interactions were considered using a zonal elution format. It was found that there was a significant increase (i.e., 2.7- to 3.6-fold) in the relative retention of several sulfonylurea drugs (i.e., acetohexamide, tolbutamide, glybenclamide and gliclazide) on columns containing normal versus glycated HSA. The addition of various long chain fatty acids to the mobile phase gave the same trend in retention for the tested drugs on both the HSA and glycated HSA columns, generally leading to lower binding. Most of the fatty acids examined produced similar or moderately different relative shifts in retention; however, palmitic acid was found to produce a much larger change in retention on columns containing glycated HSA versus normal HSA under the conditions used in this study.
Sara B G Basiaga; David S Hage
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-10-23
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  878     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-08     Completed Date:  2011-01-27     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3193-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Chemistry Department, University of Nebraska, Lincoln, Lincoln, NE 68588-0304, USA.
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MeSH Terms
Chromatography, Affinity / methods*
Diabetes Mellitus / drug therapy,  metabolism
Fatty Acids / chemistry*,  metabolism
Hypoglycemic Agents / chemistry*,  metabolism
Protein Binding
Serum Albumin / chemistry*,  metabolism
Sulfonylurea Compounds / chemistry*,  metabolism
Grant Support
Reg. No./Substance:
0/Fatty Acids; 0/Hypoglycemic Agents; 0/Serum Albumin; 0/Sulfonylurea Compounds

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