| Chromatographic investigation of macromolecular affinity interactions. | |
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MedLine Citation:
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PMID: 15782953 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High-performance monolithic disk affinity chromatography was applied to the investigation of formation of complexes between (1) complementary polyriboadenylic and polyribouridylic acids, e.g. poly(A) and poly(U), respectively, (2) poly(A) and synthetic polycation poly(allylamine), pAA. Polyriboadenylic acid and poly(allylamine) were immobilized on macroporous disks (CIM disks). Quantitative parameters of affinity interactions between macromolecules were established using frontal analysis at different flow rates. |
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Authors:
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Galina A Platonova; Tatiana B Tennikova |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1065 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-03-23 Completed Date: 2005-07-08 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 75-81 Citation Subset: IM |
Affiliation:
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Institute of Macromolecular Compounds, Russian Academy of Sciences, St Petersburg 199004, Russia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Chromatography, Affinity / methods* Chromatography, High Pressure Liquid / methods* Kinetics Poly A / chemistry* Poly U / chemistry* |
| Chemical | |
Reg. No./Substance:
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24937-83-5/Poly A; 27416-86-0/Poly U |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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