Document Detail

Chromatographic biopanning for the selection of peptides with high specificity to Pb2+ from phage displayed peptide library.
MedLine Citation:
PMID:  20709321     Owner:  NLM     Status:  MEDLINE    
Toxic heavy metal pollution is a global problem occurring in air, soil as well as water. There is a need for a more cost effective, renewable remediation technique, but most importantly, for a recovery method that is selective for one specific metal of concern. Phage display technology has been used as a powerful tool in the discovery of peptides capable of exhibiting specific affinity to various metals or metal ions. However, traditional phage display is mainly conducted in batch mode, resulting in only one equilibrium state hence low-efficiency selection. It is also unable to monitor the selection process in real time mode. In this study, phage display technique was incorporated with chromatography procedure with the use of a monolithic column, facilitating multiple phage-binding equilibrium states and online monitoring of the selection process in search of affinity peptides to Pb2+. In total, 17 candidate peptides were found and their specificity toward Pb2+ was further investigated with bead-based enzyme immunoassay (EIA). A highly specific Pb2+ binding peptide ThrAsnThrLeuSerAsnAsn (TNTLSNN) was obtained. Based on our knowledge, this is the first report on a new chromatographic biopanning method coupled with monolithic column for the selection of metal ion specific binding peptides. It is expected that this monolith-based chromatographic biopanning will provide a promising approach for a high throughput screening of affinity peptides cognitive of a wide range of target species.
Rui Nian; Duck Sang Kim; Thuong Nguyen; Lihan Tan; Chan-Wha Kim; Ik-Keun Yoo; Woo-Seok Choe
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-23
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1217     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-30     Completed Date:  2010-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  5940-9     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, South Korea.
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MeSH Terms
Amino Acid Sequence
Chromatography, Affinity / methods*
Environmental Pollutants / metabolism*
Immunoenzyme Techniques / methods*
Lead / chemistry,  metabolism*
Molecular Sequence Data
Peptide Library*
Peptides / chemistry,  isolation & purification,  metabolism*
Protein Binding
Viral Proteins / chemistry,  isolation & purification,  metabolism
Reg. No./Substance:
0/Environmental Pollutants; 0/Peptide Library; 0/Peptides; 0/Viral Proteins; 7439-92-1/Lead

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