| Chromatographic analysis of sugars in physiological fluids by postcolumn reaction with cuprammonium: a new and highly sensitive method. | |
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MedLine Citation:
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PMID: 6846816 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new highly sensitive method of high-performance liquid chromatographic analysis has been used to separate mono-, di-, and oligosaccharides present in jejunal aspirates from experimentally perfused animals. The sugars in the column eluant are detected at 280 to 310 nm after reaction with cuprammonium. No reaction coil is required between the column and uv detector because the reaction is virtually instantaneous. This technique is more sensitive than differential refractometry, so that it is possible to detect 2.5 nmol (450 ng) of glucose routinely, compared to a minimum detection limit of 100 nmol (18 micrograms) using a commercial refractive index detector. Using a suitable postcolumn pump, the technique is not sensitive to changes in solvent composition, as with gradient elution, and is applicable to analysis of other cis-cis glycols. |
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Authors:
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G K Grimble; H M Barker; R H Taylor |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 128 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1983 Feb |
Date Detail:
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Created Date: 1983-06-10 Completed Date: 1983-06-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 422-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ammonia Animals Carbohydrates / analysis*, isolation & purification Chromatography, High Pressure Liquid / methods Copper Copper Sulfate Indicators and Reagents* Intestine, Small / metabolism Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Indicators and Reagents; 7440-50-8/Copper; 7664-41-7/Ammonia; 7758-98-7/Copper Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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