Document Detail


Chromatin topology during the transformation of the mouse sperm nucleus into pronucleus in vivo.
MedLine Citation:
PMID:  3392516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Time relationships of sperm chromatin dispersion and sperm nucleoprotein replacement have been studied in vivo, by an in situ cytochemical approach. We used the Feulgen reaction to reveal DNA, which allow us to record both processes simultaneously, on the basis of the return after fertilization to haploid Feulgen values after sperm nucleoprotein replacement with somatic histones. We have shown that sperm nucleoprotein replacement occurs at around anaphase II, whereas sperm chromatin dispersion is massive between the anaphase and telophase II oocyte phases. The morphological pattern of sperm chromatin dispersion supports the idea that the process involves the whole sperm chromatin mass simultaneously, with the region located between the implantation fossa and the postacrosomial region the last to swell.
Authors:
S Garagna; C A Redi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  246     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-08-23     Completed Date:  1988-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  187-93     Citation Subset:  IM    
Affiliation:
Dipartimento di Biologia Animale, CNR, Universita' di Pavia, Italy.
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MeSH Terms
Descriptor/Qualifier:
Anaphase
Animals
Cell Nucleus / physiology*
Chromatin / physiology*
Coloring Agents
Cytophotometry
DNA / analysis
Female
Histocytochemistry
Histones / metabolism
Male
Mice
Nucleoproteins / metabolism*
Oocytes / physiology
Rosaniline Dyes*
Spermatozoa / physiology*,  ultrastructure
Telophase
Time Factors
Chemical
Reg. No./Substance:
0/Chromatin; 0/Coloring Agents; 0/Feulgen stain; 0/Histones; 0/Nucleoproteins; 0/Rosaniline Dyes; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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