| Chromatin structure and the cell division cycle. Actinomycin binding in synchronized HeLa cells. | |
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MedLine Citation:
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PMID: 5076779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Measurements of actinomycin-(3)H binding in synchronized HeLa cells reveal that the binding capacity of chromatin decreases progressively during the S phase despite a doubling of nuclear DNA content, reaches a minimal level during G(2) and mitosis, and then increases gradually throughout the subsequent G(1) interval. Since this pattern was evident in experiments with living cells, ethanol-fixed cells, and isolated nuclei, but not with purified DNA, the actinomycin binding profile may reflect changes in the degree of association between DNA and chromosomal proteins at different stages of the cell cycle. |
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Authors:
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T Pederson; E Robbins |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 55 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1972 Nov |
Date Detail:
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Created Date: 1972-12-04 Completed Date: 1972-12-04 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 322-7 Citation Subset: IM |
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Isotopes Cell Nucleus / metabolism Centrifugation, Density Gradient Chromatin / metabolism* Chromosomes / metabolism Colchicine DNA / metabolism DNA Replication Dactinomycin / metabolism* Deoxyribonucleases Hela Cells / metabolism* Methods Mitosis* Nucleoproteins / metabolism Protein Binding Ribonucleases Thymidine / metabolism Time Factors Tritium |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Chromatin; 0/Nucleoproteins; 10028-17-8/Tritium; 50-76-0/Dactinomycin; 50-89-5/Thymidine; 64-86-8/Colchicine; 9007-49-2/DNA; EC 3.1.-/Deoxyribonucleases; EC 3.1.-/Ribonucleases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1972 by The Rockefeller University Press Received Day: 16 Month: 3 Year: 1972 Revision Received Day: 19 Month: 6 Year: 1972 Print publication date: Day: 1 Month: 11 Year: 1972 Volume: 55 Issue: 2 First Page: 322 Last Page: 327 ID: 2108799 PubMed Id: 5076779 |
| CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells | |
| Thoru Pederson | |
| Elliott Robbins | |
| From the Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461. |
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| Dr. Pederson's present address is The Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545. |
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