Document Detail


Chromatin structure, DNA synthesis and transcription through the lifespan of human embryonic lung fibroblasts.
MedLine Citation:
PMID:  6200339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence in terminal embryonic fibroblasts of small molecular weight (MW) DNA independent of bulk DNA could be ascertained by three different techniques performed in parallel. This alteration was not artifactually induced, either by high pH and the detergent used or by the release of cellular enzymes. An increased thermolability of old chromatin was also observed. Cells with altered chromatin synthesized DNA and RNA according to a pattern similar to young type nuclei. Long-term treatment with hydrocortisone significantly increased the cell yield but did not prevent, in the late passages, the occurrence of old-type chromatin; the nucleolar filamentous masses, however, maintained a 'young' pattern. Short-term treatment induced only a moderate reversion in the appearance of chromatin lesions. Direct evidence was obtained of increased gene expression in the presence of hydrocortisone.
Authors:
F Puvion-Dutilleul; E Puvion; C Icard-Liepkalns; A Macieira-Coelho
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  151     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-05-02     Completed Date:  1984-05-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  283-98     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Division / drug effects
Cell Line
Cell Nucleolus / metabolism
Cell Nucleus / metabolism,  ultrastructure
Cell Survival*
Chromatin / analysis*,  ultrastructure
DNA / analysis,  biosynthesis*
Embryo, Mammalian
Fibroblasts
Humans
Hydrocortisone / pharmacology
Hydrogen-Ion Concentration
Lung
RNA / biosynthesis*
Temperature
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/Chromatin; 50-23-7/Hydrocortisone; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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