| Chromatin signature of embryonic pluripotency is established during genome activation. | |
| | |
MedLine Citation:
|
PMID: 20336069 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
After fertilization the embryonic genome is inactive until transcription is initiated during the maternal-zygotic transition. This transition coincides with the formation of pluripotent cells, which in mammals can be used to generate embryonic stem cells. To study the changes in chromatin structure that accompany pluripotency and genome activation, we mapped the genomic locations of histone H3 molecules bearing lysine trimethylation modifications before and after the maternal-zygotic transition in zebrafish. Histone H3 lysine 27 trimethylation (H3K27me3), which is repressive, and H3K4me3, which is activating, were not detected before the transition. After genome activation, more than 80% of genes were marked by H3K4me3, including many inactive developmental regulatory genes that were also marked by H3K27me3. Sequential chromatin immunoprecipitation demonstrated that the same promoter regions had both trimethylation marks. Such bivalent chromatin domains also exist in embryonic stem cells and are thought to poise genes for activation while keeping them repressed. Furthermore, we found many inactive genes that were uniquely marked by H3K4me3. Despite this activating modification, these monovalent genes were neither expressed nor stably bound by RNA polymerase II. Inspection of published data sets revealed similar monovalent domains in embryonic stem cells. Moreover, H3K4me3 marks could form in the absence of both sequence-specific transcriptional activators and stable association of RNA polymerase II, as indicated by the analysis of an inducible transgene. These results indicate that bivalent and monovalent domains might poise embryonic genes for activation and that the chromatin profile associated with pluripotency is established during the maternal-zygotic transition. |
| | |
Authors:
|
Nadine L Vastenhouw; Yong Zhang; Ian G Woods; Farhad Imam; Aviv Regev; X Shirley Liu; John Rinn; Alexander F Schier |
Related Documents
:
|
18375619 - Saccharomyces cerevisiae phospholipase c regulates transcription of msn2p-dependent str... 20729799 - Chromatin immunoprecipitation (chip) to assay dynamic histone modification in activated... 22343619 - A network-based, integrative study to identify core biological pathways that drive brea... 11554329 - Analyzing the contributions of chromatin structure in nuclear hormone receptor activate... 6088079 - Retrovirus-induced lethal mutation in collagen i gene of mice is associated with an alt... 16608399 - Chromatin modification and senescence: linkage by tumor suppressors? 1965189 - Tissue-specific transcription of p-450(11 beta) gene in vitro. 12589799 - Transcription of ahpc, katg, and kate genes in escherichia coli is regulated by polyami... 17093389 - Characterization of gene expression profiles of normal canine retina and brain using a ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-03-24 |
Journal Detail:
|
Title: Nature Volume: 464 ISSN: 1476-4687 ISO Abbreviation: Nature Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-04-08 Completed Date: 2010-05-17 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 0410462 Medline TA: Nature Country: England |
Other Details:
|
Languages: eng Pagination: 922-6 Citation Subset: IM |
Affiliation:
|
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GEO/GPL9970; GSE20023 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromatin / genetics*, metabolism* Chromatin Immunoprecipitation Gene Expression Regulation, Developmental* / genetics Gene Silencing Genome / genetics* Histones / chemistry, metabolism Lysine / metabolism Methylation Oligonucleotide Array Sequence Analysis Pluripotent Stem Cells / metabolism* Promoter Regions, Genetic / genetics RNA Polymerase II / metabolism Transcriptional Activation Transgenes Zebrafish / embryology*, genetics*, metabolism Zebrafish Proteins / genetics Zygote / cytology, metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
1R01 HG004069/HG/NHGRI NIH HHS; 5R01 GM56211/GM/NIGMS NIH HHS; DP1 OD003958-05/OD/NIH HHS; R01 GM056211-11/GM/NIGMS NIH HHS; R01 GM056211-12/GM/NIGMS NIH HHS; R01 GM056211-12S1/GM/NIGMS NIH HHS; R01 GM056211-13/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Chromatin; 0/Histones; 0/Zebrafish Proteins; 56-87-1/Lysine; EC 2.7.7.-/RNA Polymerase II |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The complete mitochondrial DNA genome of an unknown hominin from southern Siberia.
Next Document: Zscan4 regulates telomere elongation and genomic stability in ES cells.