Document Detail


Chromatin remodeling in plant cell culture: patterns of DNA methylation and histone H3 and H4 acetylation vary during growth of asynchronous potato cell suspensions.
MedLine Citation:
PMID:  15922608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Changes in DNA cytosine methylation and core histone multi-acetylation were determined in cell suspension cultures of potato (Solanum tuberosum L. cv. Russet Burbank) during 15 days of in vitro culture. Cell subculture induced a transient 33% decrease in genome-wide 5-methylcytosine (5mC) content and a transient threefold increase in transcription rates that were most evident at 6 and 9 days after subculture, respectively. In contrast to the global reduction in 5mC content, subculture resulted in a transient twofold increase in 5mC levels within 5'-CCGG-3' sequences and no detectable change in 5'-CG-3' methylation. Multi-acetylation of histones H3.1, H3.2 and H4 rose 2-, 1.5- and 3-fold by 9, 9 and 12 days after subculture, respectively. All observed epigenetic changes were reset during aging of cell cultures. Inclusion of the histone deacetylase inhibitor trichostatin A (TSA) and/or the cytosine methylation inhibitor 5-azacytidine (5AC) in culture sequentially decreased genome-wide 5mC levels by approximately 25% at day 9, then decreased 5'-mCmCGG-3' by 30-50% and increased H3 and H4 multi-acetylation by 30-60% at day 15, compared to controls. Treatment with 5AC or TSA alone or in combination had no effect on RNA synthesis at day 9. At day 15, 5AC treatment remained ineffective, while de novo RNA synthesis was approximately twofold higher in cells grown in both inhibitors or in TSA alone. Collectively, these results demonstrate that in potato suspension cultures, rapid, reversible changes in 5mC levels precede regulatory post-translational acetylation of core histones, and suggest that interactions between these epigenetic processes appear to be necessary to power transcription and growth induction in potato cells.
Authors:
R David Law; Jeffrey C Suttle
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology and biochemistry : PPB / Société française de physiologie végétale     Volume:  43     ISSN:  0981-9428     ISO Abbreviation:  Plant Physiol. Biochem.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-07-18     Completed Date:  2006-02-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882449     Medline TA:  Plant Physiol Biochem     Country:  France    
Other Details:
Languages:  eng     Pagination:  527-34     Citation Subset:  IM    
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Northern Crop Science Laboratory, Sugarbeet and Potato Research, Post Office Box 5677, State University Station, Fargo, ND 58105-5677, USA.
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MeSH Terms
Descriptor/Qualifier:
5-Methylcytosine / metabolism
Acetylation
Azacitidine / pharmacology
Chromatin Assembly and Disassembly*
Cytosine / metabolism
DNA Methylation*
Epigenesis, Genetic
Histones / metabolism*
Hydroxamic Acids / pharmacology
Solanum tuberosum / cytology,  genetics,  metabolism*
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Histones; 0/Hydroxamic Acids; 320-67-2/Azacitidine; 554-01-8/5-Methylcytosine; 58880-19-6/trichostatin A; 71-30-7/Cytosine

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