Document Detail

Chromatin organization and restriction endonuclease activity on human metaphase chromosomes.
MedLine Citation:
PMID:  6315311     Owner:  NLM     Status:  MEDLINE    
Human metaphase chromosomes were treated with HaeIII, HindIII, EcoRI, and AluI restriction endonucleases and subsequently stained with either Giemsa or ethidium bromide. The results obtained seemed to suggest that the structural organization of specific chromosome regions can play a primary role in determining the cytological effect after digestion with specific restriction endonucleases.
R Mezzanotte; U Bianchi; R Vanni; L Ferrucci
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  36     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1983  
Date Detail:
Created Date:  1984-01-27     Completed Date:  1984-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  562-6     Citation Subset:  IM    
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MeSH Terms
Azure Stains
Chromatin / analysis*
Chromosome Banding
Chromosomes, Human / analysis*
DNA Restriction Enzymes / metabolism*
Deoxyribonuclease EcoRI
Deoxyribonuclease HindIII
Deoxyribonucleases, Type II Site-Specific*
Heterochromatin / analysis
Reg. No./Substance:
0/Azure Stains; 0/Chromatin; 0/Heterochromatin; 3546-21-2/Ethidium; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/Deoxyribonuclease EcoRI; EC 3.1.21.-/Deoxyribonuclease HindIII; EC 3.1.21.-/endodeoxyribonuclease AluI; EC, Type II Site-Specific; EC type II deoxyribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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