| Chromatin insulation by a transcriptional activator. | |
| | |
MedLine Citation:
|
PMID: 12547916 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In eukaryotic genomes, transcriptionally active regions are interspersed with silent chromatin that may repress genes in its vicinity. Chromatin insulators are elements that can shield a locus from repressive effects of flanking chromatin. Few such elements have been characterized in higher eukaryotes, but transcriptional activating elements are an invariant feature of active loci and have been shown to suppress transgene silencing. Hence, we have assessed the ability of a transcriptional activator to cause chromatin insulation, i.e., to relieve position effects at transgene integration sites in cultured cells. The transgene contained a series of binding sites for the metal-inducible transcriptional activator MTF, linked to a GFP reporter. Clones carrying single integrated transgenes were derived without selection for expression, and in most clones the transgene was silent. Induction of MTF resulted in transition of the transgene from the silent to the active state, prolongation of the active state, and a marked narrowing of the range of expression levels at different genomic sites. At one genomic site, prolonged induction of MTF resulted in suppression of transgene silencing that persisted after withdrawal of the induction stimulus. These results are consistent with MTF acting as a chromatin insulator and imply that transcriptional activating elements can insulate active loci against chromatin repression. |
| | |
Authors:
|
Nathan B Sutter; David Scalzo; Steven Fiering; Mark Groudine; David I K Martin |
Related Documents
:
|
8415626 - Testis-specific expression of a metallothionein i-driven transgene correlates with unde... 9374406 - Gata-1 expression pattern can be recapitulated in living transgenic zebrafish using gfp... 10468616 - Long-term expression of human coagulation factor viii and correction of hemophilia a af... 9016796 - Adenovirus-mediated inducible gene expression through tetracycline-controllable transac... 11089536 - Differential transcriptional regulation of rat vasopressin gene expression by estrogen ... 7507966 - Decay-accelerating factor on human umbilical vein endothelial cells. its histamine-indu... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. Date: 2003-01-23 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 100 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2003 Feb |
Date Detail:
|
Created Date: 2003-02-05 Completed Date: 2003-03-18 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 1105-10 Citation Subset: IM |
Affiliation:
|
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line Cell Separation Chromatin / metabolism* DNA-Binding Proteins Dose-Response Relationship, Drug Flow Cytometry Genes, Reporter Green Fluorescent Proteins Humans K562 Cells Luminescent Proteins / metabolism Plasmids / metabolism Protein Structure, Tertiary Time Factors Transcription Factors / metabolism Transcriptional Activation* Transgenes |
| Grant Support | |
ID/Acronym/Agency:
|
HL478790/HL/NHLBI NIH HHS; HL57620/HL/NHLBI NIH HHS; HL65440/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Chromatin; 0/DNA-Binding Proteins; 0/Luminescent Proteins; 0/Transcription Factors; 0/transcription factor MTF-1; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ecological community description using the food web, species abundance, and body size.
Next Document: A role for Mints in transmitter release: Mint 1 knockout mice exhibit impaired GABAergic synaptic tr...