| Chromatin of Trypanosoma cruzi: in situ analysis revealed its unusual structure and nuclear organization. | |
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MedLine Citation:
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PMID: 11968019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chromatin of Trypanosoma cruzi is known to be organized in classical nucleosomal filaments, but surprisingly, these filaments do not fold in visible chromosomes and the nuclear envelope is preserved during cell division. Our hypothesis about the role of chromatin structure in regulating gene expression and, more generally, cell functioning, pressed us to verify if chromatin organization is modulated during the parasite life-cycle. To this end, we analyzed in situ the fine structural organization of T. cruzi chromatin by means of an integrated biophysical approach, using differential scanning calorimetry and fluorescence microscopy. We observed that logarithmic forms exhibit a less condensed chromatin with respect to the stationary ones. Thermal analysis revealed that parasite chromatin is organized in three main levels of condensation, barring from the polynucleosomal filament till to superstructured fibers. Besides, the fluorescence images of nuclei showed a characteristic chromatin distribution, with defined domains localized near to the nuclear envelope. While in stationary parasites, these regions are highly condensed, in logarithmic forms they unfold by extending themselves toward the center of nucleus. These observations suggest that, in comparison with higher eukaryotes, in T. cruzi the nuclear envelope plays an unusual and pivotal role in interphase and in mitosis. |
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Authors:
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Barbara Spadiliero; Claudio Nicolini; Giancarlo Mascetti; Diana Henríquez; Laura Vergani |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 85 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-05-23 Completed Date: 2002-10-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 798-808 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Wiley-Liss, Inc. |
Affiliation:
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Department of Cell Biology, Simón Bolívar University, Caracas 89 000, Venezuela. bspadi@usb.ve |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calorimetry, Differential Scanning Cell Nucleus / ultrastructure Chromatin / chemistry*, ultrastructure* Image Processing, Computer-Assisted Microscopy, Electron Microscopy, Fluorescence Trypanosoma cruzi / chemistry*, growth & development, ultrastructure* |
| Chemical | |
Reg. No./Substance:
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0/Chromatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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