Document Detail

Chromaffin cell death induced by 6-hydroxydopamine is independent of mitochondrial swelling and caspase activation.
MedLine Citation:
PMID:  12603830     Owner:  NLM     Status:  MEDLINE    
Our results provide evidence that 6-hydroxydopamine induced, after auto-oxidation, toxic levels of hydrogen peroxide (H2O2) that caused bovine chromaffin cell toxicity and death. 6-Hydroxydopamine (6-OHDA) treatment markedly reduced, in a dose-response fashion, chromaffin cell viability. Cell death was accompanied by cell shrinkage, nuclear condensation and DNA degradation. Under our experimental conditions, 6-OHDA auto-oxidation formed quinones and reactive oxygen species (ROS) that mainly contributed to 6-OHDA-induced cytotoxicity in bovine chromaffin cells. Accordingly, different antioxidants, including catalase, vitamin E, Mn(IIItetrakis(4-benzoic acid)porphyrin chloride (MnTBAP) or ascorbic acid, provided protection against 6-OHDA-induced toxicity. Further evidence that 6-OHDA induces oxidative stress is provided by the fact that this compound decreased total mitochondrial reduced NAD(P)H levels. Our results also suggest that mitochondrial swelling and caspase activation do not play a direct role in 6-OHDA-induced death in bovine chromaffin cells.
María F Galindo; Joaquín Jordán; Carmen González-García; Valentín Ceña
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  84     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-02-26     Completed Date:  2003-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1066-73     Citation Subset:  IM    
Centro Regional de Investigaciones Biomédicas y Dpto. de Ciencias Médicas, Facultad de Medicina, Universidad de Castilla-La Mancha, Avda. España, s/n, 02071 Albacete, Spain.
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MeSH Terms
Antioxidants / pharmacology
Caspases / metabolism*
Cells, Cultured
Chromaffin Cells / cytology,  drug effects*,  enzymology*
Dose-Response Relationship, Drug
Hydrogen Peroxide / metabolism
Mitochondria / drug effects*,  metabolism
NAD / metabolism
NADP / metabolism
Oxidation-Reduction / drug effects
Oxidopamine / metabolism,  toxicity*
Quinones / metabolism
Reactive Oxygen Species / metabolism
Reg. No./Substance:
0/Antioxidants; 0/Quinones; 0/Reactive Oxygen Species; 1199-18-4/Oxidopamine; 53-59-8/NADP; 53-84-9/NAD; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/Caspases

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