Document Detail


Chorionic peptidase inactivates GnRH as a post-proline peptidase.
MedLine Citation:
PMID:  1502138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, we have described a chorionic peptidase (C-ase-1) which inactivates gonadotropin releasing hormone (GnRH), oxytocin, angiotensin II and thyrotropin releasing hormone. Since all these hormones contain a proline residue, we proposed that C-ase-1 may act as a post-proline peptidase. Using HPLC and amino acid analyses, we have defined the products which resulted from enzymatic inactivation of GnRH by C-ase-1. The N-terminal nonapeptide of GnRH was isolated by HPLC and confirmed by amino acid composition analyses. Thus, it was demonstrated that C-ase-1 acts as a post-proline peptidase when inactivating GnRH, yielding the nonapeptide, i.e., des-Gly10-NH2-GnRH, and Gly-NH2. The levels of intrauterine GnRH, angiotensin II, oxytocin and thyrotropin releasing hormone may be affected and integrated by this enzyme. Thus, C-ase-1 may play an important role in the regulation of the paracrine and endocrine function during pregnancy.
Authors:
I S Kang; T M Siler-Khodr
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Placenta     Volume:  13     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:    1992 Jan-Feb
Date Detail:
Created Date:  1992-09-17     Completed Date:  1992-09-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  81-7     Citation Subset:  IM    
Affiliation:
Department of Obstetrics & Gynecology, University of Texas Health Science Center, San Antonio 78284.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Chromatography, High Pressure Liquid
Endopeptidases / pharmacology*
Gonadotropin-Releasing Hormone / metabolism*
Humans
Radioimmunoassay
Grant Support
ID/Acronym/Agency:
HD 10202/HD/NICHD NIH HHS; HD 21708/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 33515-09-2/Gonadotropin-Releasing Hormone; EC 3.4.-/Endopeptidases; EC 3.4.99.-/chorionic peptidase I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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