Document Detail


Choriocarcinoma-like human chorionic gonadotrophin (HCG) and HCG bioactivity during the first trimester of pregnancy.
MedLine Citation:
PMID:  11006201     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to evaluate the distribution of choriocarcinoma-like human chorionic gonadotrophin (HCG) isoforms during first trimester pregnancy and their relationship with in-vitro HCG bioactivity. This was done by means of a retrospective analysis of patients' sera with first trimester normal intrauterine and abnormal (ectopic) pregnancies. Serum samples were obtained from 38 women with an amenorrhoea of <10 weeks. From these, 19 had a normal intrauterine pregnancy (IUP) and 19 an ectopic pregnancy (EP). Total immunoreactive HCG (HCGi), free beta-HCGi and oestradiol were measured by enzyme immunoassays and bioactive HCG by the mouse Leydig cell bioassay. The alterations in HCG isoform content were measured by the combination of two immunometric assays, B152 for choriocarcinoma-like HCG and B109 for intact HCG detection and expressed as the B152/B109 ratio. Choriocarcinoma-like HCG isoforms ratio measured by B152 and B109 assays was significantly higher in the low subgroups of free beta-HCGi and gestational age (P = 0.0111 and 0.0036 respectively). Whereas bioactive to immunoreactive HCG ratios (b/i ratio) were significantly higher when free beta-HCGi concentrations were low (P = 0.0010), no correlation was found between the variation of bioactivity (b/i ratio) and the proportion of choriocarcinoma-like HCG isoforms (B159/B108). It is concluded that in first trimester pregnancies (i) the modulation of HCG in-vitro bioactivity is not related to the variation of choriocarcinoma-like HCG isoforms secretion and (ii) the amount of choriocarcinoma-like HCG isoforms secreted by the early trophoblast is predominant and may be the result of an early developmental regulation of glycosylation enzyme.
Authors:
P Mock; G Kovalevskaya; J F O'Connor; A Campana
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  15     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-12-13     Completed Date:  2000-12-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2209-14     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, University Hospital, Geneva, Switzerland. Pascal.Mock@hcuge.ch
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MeSH Terms
Descriptor/Qualifier:
Amenorrhea / blood
Animals
Biological Assay / methods
Chorionic Gonadotropin / blood*,  immunology,  metabolism
Female
Humans
Leydig Cells / immunology,  metabolism
Male
Mice
Pregnancy
Pregnancy Trimester, First / blood*
Pregnancy, Ectopic / blood*
Protein Isoforms / blood,  metabolism
Retrospective Studies
Grant Support
ID/Acronym/Agency:
ESO7589/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Protein Isoforms; 0/glycosylated HCG

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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