| Choosing Orientation: Influence of Cargo Geometry and ActA Polarization on Actin Comet Tails. | |
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MedLine Citation:
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PMID: 22219381 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Networks of polymerizing actin filaments can propel intracellular pathogens and drive movement of artificial particles in reconstituted systems. While biochemical mechanisms activating actin network assembly have been well characterized, it remains unclear how particle geometry and large-scale force balance affect emergent properties of movement. We reconstituted actin-based motility using ellipsoidal beads resembling the geometry of Listeria monocytogenes. Beads coated uniformly with the L. monocytogenes ActA protein migrated equally well in either of two distinct orientations, with their long axes parallel or perpendicular to the direction of motion, while intermediate orientations were unstable. When beads were coated with a fluid lipid bilayer rendering ActA laterally mobile, beads predominantly migrated with their long axes parallel to the direction of motion, mimicking the orientation of motile L. monocytogenes. Generating an accurate biophysical model to account for our observations required the combination of elastic propulsion and tethered-ratchet actin polymerization theories. Our results indicate that the characteristic orientation of L. monocytogenes must be due to polarized ActA rather than intrinsic actin network forces. Furthermore, viscoelastic stresses, forces and torques produced by individual actin filaments, and lateral movement of molecular complexes must all be incorporated to correctly predict large-scale behavior in the actin-based movement of non-spherical particles. |
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Authors:
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Catherine I Lacayo; Paula A G Soneral; Jie Zhu; Mark A Tsuchida; Matthew J Footer; Frederick S Soo; Yu Lu; Younan Xia; Alexander Mogilner; Julie A Theriot |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-4 |
Journal Detail:
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Title: Molecular biology of the cell Volume: - ISSN: 1939-4586 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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†Department of Biochemistry, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA ‡Department of Microbiology and Immunology, and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA §Department of Neurobiology, Physiology and Behavior and Department of Mathematics, University of California, Davis, CA ¶Department of Materials Science and Engineering, and Department of Chemistry, University of Washington, Seattle, WA #currently at Department of Biomedical Engineering, Washington University, St. Louis, MO. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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