Document Detail


Chondroitin sulfate acts in concert with semaphorin 3A to guide tangential migration of cortical interneurons in the ventral telencephalon.
MedLine Citation:
PMID:  20071458     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chondroitin sulfate (CS) carrying proteoglycans (PGs) are widely expressed in the nervous system, and there is increasing evidence that they regulate developmental mechanisms like neurite outgrowth, axonal guidance and neuronal migration. Moreover, they can also act indirectly by organizing and/or modulating growth factors and guidance molecules. We found that chondroitin-4-sulfate is coexpressed with semaphorin 3A (Sema 3A) in the striatal mantle zone (SMZ), a nontarget region of neuropilin (Nrp)-1-expressing cortical interneurons flanking their migratory route in the subpallium. Using in vitro assays, we showed that CS PGs exert a repulsive effect on cortical interneurons, independently of Sema 3A, due to the CS side chains. We further showed that extracellular Sema 3A binds to CS. Disrupting Sema 3A-Nrp-1 signaling led migrating medial ganglionic eminence neurons to inappropriately invade the SMZ and even more so after removal of the CS side chains. Moreover, we found that soluble Sema 3A enhances the CS-induced repulsion in vitro. We concluded that CS acts as a repellent for cortical interneurons and that, in addition, CS restricts secreted Sema 3A within SMZ. Thus, both molecules act in concert to repel cortical interneurons from the SMZ during tangential migration toward the cerebral cortex.
Authors:
Geraldine Zimmer; Sheine M Schanuel; Susanne Bürger; Franco Weth; André Steinecke; Jürgen Bolz; Roberto Lent
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-13
Journal Detail:
Title:  Cerebral cortex (New York, N.Y. : 1991)     Volume:  20     ISSN:  1460-2199     ISO Abbreviation:  Cereb. Cortex     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-13     Completed Date:  2010-12-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9110718     Medline TA:  Cereb Cortex     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2411-22     Citation Subset:  IM    
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, CEP 21941-902, Brazil. g.zimmer@uni-jena.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement / drug effects,  physiology*
Cerebral Cortex / cytology*
Chondroitin ABC Lyase / pharmacology
Chondroitin Sulfate Proteoglycans / genetics,  metabolism
Chondroitin Sulfates / metabolism*
Corpus Striatum / metabolism*
Embryo, Mammalian
Gene Expression Regulation, Developmental / drug effects,  physiology
Green Fluorescent Proteins / genetics
Interneurons / drug effects,  physiology*
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Confocal / methods
Neuropilin-1 / metabolism
Organ Culture Techniques
Semaphorin-3A / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Chondroitin Sulfate Proteoglycans; 0/Semaphorin-3A; 0/enhanced green fluorescent protein; 144713-63-3/Neuropilin-1; 147336-22-9/Green Fluorescent Proteins; 9007-28-7/Chondroitin Sulfates; EC 4.2.2.20/Chondroitin ABC Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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