Document Detail

Chondrocyte viability in fresh and frozen large human osteochondral allografts: effect of cryoprotective agents.
MedLine Citation:
PMID:  17954166     Owner:  NLM     Status:  MEDLINE    
Chondrocyte survival is a major goal for the effective storage and clinical performance of human osteochondral allografts. The majority of animal and human cryopreservation studies conducted so far have been performed in small osteochondral cylinders. Using human tibial plateaus as a model for large osteochondral pieces, this work sought to evaluate the cryoprotective efficiency of glycerol and dimethylsulfoxide (DMSO), and to identify cryopreservation conditions suitable for use in tissue banks. Human tibial plateaus harvested from 7 cadaveric tissue donors were incubated in the presence or absence of cryoprotective agents (CPA): 10% or 15% glycerol and 10% DMSO in a Ham F-12 nutrient mixture. Chondrocyte viability was assessed immediately after thawing, using the MTT reduction assay and a fluorescence microscopic method. The tibial plateaus frozen in the absence of CPA showed a significant decrease in chondrocyte viability. The use of CPA significantly increased chondrocyte viability compared with cartilage frozen without CPA (nearly 50% versus 80% living chondrocytes with 10% glycerol versus 10% DMSO, respectively) relative to that in fresh cartilage. In this regard, 10% DMSO was slightly more effective than either 10% or 15% glycerol, eliciting the recovery of approximately 15% relative to the living chondrocyte content in fresh cartilage. In all conditions, fluorescence microscopic studies showed that surviving chondrocytes were restricted to the superficial cartilage layer. Human tibial plateaus seemed to be a good experimental model to establish cryopreservation methods applicable to large human osteochondral pieces in tissue banks.
F Judas; S Rosa; L Teixeira; C Lopes; A Ferreira Mendes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transplantation proceedings     Volume:  39     ISSN:  0041-1345     ISO Abbreviation:  Transplant. Proc.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-23     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243532     Medline TA:  Transplant Proc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2531-4     Citation Subset:  IM    
Orthopaedics Department of Coimbra University Hospitals and Faculty of Medicine, University of Coimbra, 3000 Coimbra, Portugal.
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MeSH Terms
Bone Transplantation / physiology*
Cartilage, Articular / injuries,  transplantation*
Cell Survival
Chondrocytes / cytology*,  drug effects,  transplantation*
Cryoprotective Agents / therapeutic use*
Tibia / transplantation
Tissue Banks
Tissue Donors
Transplantation, Homologous
Reg. No./Substance:
0/Cryoprotective Agents

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