| Cholinesterase activity and mRNA level of nicotinic acetylcholine receptors (alpha4 and beta2 Subunits) in blood of elderly Chinese diagnosed as Alzheimer's disease. | |
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MedLine Citation:
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PMID: 20157241 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the study is to investigate the cholinergic deficit in Alzheimer's disease (AD) and identify candidate blood biomarkers for the diagnosis of the disease. Twenty-nine elderly Chinese diagnosed with AD and 33 age-matched controls were selected. The activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) in plasma were detected by a spectrophotometric method, and the mRNA levels of alpha4 and beta2 nicotinic acetylcholine receptor (nAChR) subunits in blood leukocytes were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that AChE activity in plasma was significantly lower in the AD group than in normal controls, while BuChE activity did not show any differences between AD and controls; mRNA levels of both alpha4 and beta2 nAChR subunits in blood leukocytes were significantly lower in the AD group than in controls. The AChE activity and the mRNA levels of alpha4 and beta2 nAChR subunits in the AD patients were also significantly correlated with cognitive test scores. No differences of AChE in plasma or alpha4 and beta2 nAChR subunits in blood leukocytes were detected between smoking and non-smoking subjects. The results indicated that the decreases in the activity of AChE and in the mRNA levels of nAChR alpha4 and beta2 subunits from the peripheral blood of patients with AD might serve as supplementary indicators for the clinical diagnosis of AD. |
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Authors:
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Lan-Jian Zhang; Yan Xiao; Xiao-Lan Qi; Ke-Ren Shan; Jin-Jing Pei; Shi-Xiang Kuang; Fang Liu; Zhi-Zhong Guan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of Alzheimer's disease : JAD Volume: 19 ISSN: 1875-8908 ISO Abbreviation: J. Alzheimers Dis. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-02-16 Completed Date: 2010-05-05 Revised Date: 2010-06-01 |
Medline Journal Info:
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Nlm Unique ID: 9814863 Medline TA: J Alzheimers Dis Country: Netherlands |
Other Details:
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Languages: eng Pagination: 849-58 Citation Subset: IM |
Affiliation:
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Department of Pathology and Molecular Biology, Guiyang Medical University, Guiyang, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Alzheimer Disease* / blood, ethnology, genetics Asian Continental Ancestry Group / ethnology* Butyrylcholinesterase Cholinesterases / blood* Cognition Disorders / blood, diagnosis, genetics Cyclophilins / genetics Female Humans Male Middle Aged Neuropsychological Tests RNA, Messenger / biosynthesis, genetics* Receptors, Nicotinic / blood*, genetics, metabolism Severity of Illness Index |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Receptors, Nicotinic; 0/nicotinic acetylcholine receptor alpha4 subunit; EC 3.1.1.-/Butyrylcholinesterase; EC 3.1.1.8/Cholinesterases; EC 5.2.1.-/Cyclophilins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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