Document Detail


Cholinergic differentiation of clonal rat pheochromocytoma cells (PC12) induced by factors contained in glioma-conditioned medium: enhancement of high-affinity choline uptake system and reduction of norepinephrine uptake system.
MedLine Citation:
PMID:  3948005     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of glioma-conditioned medium (GCM) and factors contained in GCM on the neurochemical differentiation of the PC12 clone of rat pheochromocytoma cells were investigated. The results obtained are as follows. The accumulation of choline into PC12 cells proceeded through two uptake systems with high (Km = 3.20 microM) and low (Km = 65.2 muM) affinities as revealed by least-squares iterative fitting of a substrate-velocity curve to the data. Culturing of PC12 cells in the presence of GCM led to a 5-fold increase in the Vmax value of the high-affinity uptake system without affecting the Km of the high-affinity uptake system. Both Km and Vmax of the low-affinity uptake system were unaffected by the GCM treatment. The high-affinity choline uptake system in both GCM-treated and untreated PC12 cells was devoid of Na+ dependency and showed low sensitivity to hemicholinium-3. The ratio of [3H]acetylcholine converted from [3H]choline taken up by PC12 cells at 1 muM choline for 1 h was two-fold higher than that by untreated cells. PC12 possess a high-affinity norepinephrine uptake system. Culturing of PC12 cells in the presence of GCM led to a decrease in the rate of uptake of 3 muM norepinephrine to 43% of that in control cells. The 40-K and 10-K fractions isolated by gel filtration of GCM had both abilities to enhance the high-affinity choline uptake system and to suppress the high-affinity norepinephrine uptake system. From these observations it was concluded that GCM contains factors which induce the cholinergic neuronal differentiation of PC12 cells.
Authors:
I Matsuoka; R Satake; K Kurihara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Brain research     Volume:  389     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-04-07     Completed Date:  1986-04-07     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  145-52     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / metabolism
Adrenal Gland Neoplasms / metabolism*
Animals
Cell Differentiation / drug effects
Choline / metabolism*
Cholinergic Fibers / metabolism
Clone Cells / metabolism,  pathology
Culture Media / analysis
Glioma / metabolism*
Hemicholinium 3 / pharmacology
Kinetics
Nerve Growth Factors / physiology
Nerve Tissue Proteins / physiology*
Norepinephrine / metabolism*
Pheochromocytoma / metabolism*
Rats
Sodium / metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 312-45-8/Hemicholinium 3; 51-41-2/Norepinephrine; 51-84-3/Acetylcholine; 62-49-7/Choline; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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