| Choline incorporation into phospholipids in mesothelial cells in vitro. | |
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MedLine Citation:
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PMID: 8241329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the effect of extracellular choline concentration on phospholipid production and handling by peritoneal mesothelial cells in vitro. DESIGN AND MEASUREMENTS: Radiolabeled choline was used to monitor the formation of phosphatidylcholine (PC), sphingomyelin (SPH), and lysophosphatidylcholine (LPC) by rat and rabbit mesothelial cells as a function of concentration and time of exposure to choline. The subcellular location of the newly formed phospholipids was examined by ultracentrifugation in Percoll-sucrose gradients using analytical cell fractionation techniques. The fatty acid composition of the PC formed was determined by thin-layer chromatography (TLC) and gas chromatography. RESULTS: Choline incorporation into PC, SPH, and LPC increased with extracellular choline levels up to 640 mumol/L, which is 100 times greater than physiological levels of choline in plasma and 20 times higher than choline levels measured in peritoneal dialysis effluent. The newly formed, radiolabeled phospholipids were primarily found in a single subcellular compartment that exhibited a buoyant density of 1.05 g/mL in Percoll-sucrose gradients. Analysis of the fatty acyl groups of PC obtained from the mesothelial cells showed enrichment in palmitic [16:0], oleic [18:1], and linoleic [18:2] acids. CONCLUSION: The rate of phospholipid formation by mesothelial cells in vitro can be manipulated, in part, by choline concentration. |
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Authors:
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J T Hjelle; K R Steidley; T M Pavlina; J W Dobbie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis Volume: 13 ISSN: 0896-8608 ISO Abbreviation: Perit Dial Int Publication Date: 1993 |
Date Detail:
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Created Date: 1994-01-06 Completed Date: 1994-01-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8904033 Medline TA: Perit Dial Int Country: CANADA |
Other Details:
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Languages: eng Pagination: 289-95 Citation Subset: IM |
Affiliation:
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Department of Basic Sciences, University of Illinois College of Medicine at Peoria 61656. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Radioisotopes / diagnostic use Cell Fractionation Cell Separation Cells, Cultured Choline / metabolism*, pharmacokinetics Epithelial Cells Epithelium / chemistry, metabolism Fatty Acids / analysis Lysophosphatidylcholines / analysis, biosynthesis Mice Peritoneum / chemistry, cytology, metabolism* Phosphatidylcholines / analysis, biosynthesis Phospholipids / analysis, biosynthesis* Rabbits Rats Sphingomyelins / analysis, biosynthesis Tritium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Fatty Acids; 0/Lysophosphatidylcholines; 0/Phosphatidylcholines; 0/Phospholipids; 0/Sphingomyelins; 10028-17-8/Tritium; 62-49-7/Choline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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