| Cholesterol side-chain cleavage cytochrome P450 and 3beta-hydroxysteroid dehydrogenase expression and the concentrations of steroid hormones in the follicular fluids of different phenotypes of healthy and atretic bovine ovarian follicles. | |
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MedLine Citation:
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PMID: 12930727 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bovine ovarian antral follicles exhibit either one or the other of two patterns of granulosa cell death in atresia. Death can commence either from the antrum and progress toward the basal lamina (antral atresia) or the converse (basal atresia). In basal atresia, the remaining live antrally situated cells appeared to continue maturing. Beyond that, little is known about these distinct patterns of atresia. Healthy (nonatretic) follicles also exhibit either one or the other of two patterns of granulosa cell shape, follicular basal lamina ultrastructure or location of younger cells within the membrana granulosa. To examine these different phenotypes, the expression of the steroidogenic enzymes cholesterol side-chain cleavage cytochrome P450 (SCC) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) in granulosa cells and concentrations of steroid hormones in follicular fluid were measured in individual histologically classified bovine antral follicles. Healthy follicles first expressed SCC and 3beta-HSD in granulosa cells only when the follicles reached an approximate threshold of 10 mm in diameter. The pattern of expression in antral atretic follicles was the same as healthy follicles. Basal atretic follicles were all <5 mm. In these, the surviving antral granulosa cells expressed SCC and 3beta-HSD. In examining follicles of 3-5 mm, basal atretic follicles were found to have substantially elevated progesterone (P < 0.001) and decreased androstenedione and testosterone compared to healthy and antral atretic follicles. Estradiol was highest in the large healthy follicles, lower in the small healthy follicles, lower still in the antral atretic follicles, and lowest in the basal atretic follicles. Our findings have two major implications. First, the traditional method of identifying atretic follicles by measurement of steroid hormone concentrations may be less valid with small bovine follicles. Second, features of the two forms of follicular atresia are so different as to imply different mechanisms of initiation and regulation. |
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Authors:
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Helen F Irving-Rodgers; Malgorzata Krupa; Raymond J Rodgers |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-08-20 |
Journal Detail:
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Title: Biology of reproduction Volume: 69 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-11-21 Completed Date: 2004-08-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
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Languages: eng Pagination: 2022-8 Citation Subset: IM |
Affiliation:
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Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, University of Adelaide, Adelaide, South Australia 5005, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-Hydroxysteroid Dehydrogenases
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metabolism* Androstenedione / metabolism Animals Cattle Cholesterol Side-Chain Cleavage Enzyme / metabolism* Estradiol / metabolism Female Follicular Atresia Follicular Fluid / metabolism* Ovarian Follicle / metabolism*, pathology, physiology Progesterone / metabolism Reference Values Steroids / metabolism* Testosterone / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Steroids; 50-28-2/Estradiol; 57-83-0/Progesterone; 58-22-0/Testosterone; 63-05-8/Androstenedione; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.14.15.6/Cholesterol Side-Chain Cleavage Enzyme |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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