|Cholesterol metabolism in the macrophage. 3. Ingestion and intracellular fate of cholesterol and cholesterol esters.|
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|PMID: 4550608 Owner: NLM Status: MEDLINE|
|Phagocytosis of cholesterol-containing particles resulted in the formation of an intralysosomal cholesterol compartment. Cholesterol was excreted out of the macrophage with a single exponential rate which depended on the concentration of acceptor lipoproteins in the medium. Exchange kinetics performed on cells which had ingested particulate cholesterol suggested that excretion occurred by the same mechanism as exchange. Cholesterol esters as particulate albumin coacervates were taken up by macrophages and hydrolyzed by a lysosomal cholesterol esterase with optimal activity at pH 4.0. Cholesteryl linoleate was hydrolyzed much more readily than cholesteryl palmitate. The amount of cholesterol esterase and its specific activity increased during the in vitro cultivation of macrophages. Intralysosomally, cholesteryl linoleate and palmitate were hydrolyzed to free cholesterol which was excreted from the macrophage and recovered in the medium. Since cholesteryl linoleate was hydrolyzed more rapidly than free cholesterol was excreted into the medium, free cholesterol accumulated intralysosomally. Cholesteryl palmitate was hydrolyzed more slowly, and the rate of hydrolysis was limiting for excretion of the free cholesterol from within the lysosome.|
|Z Werb; Z A Cohn|
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|Type: In Vitro; Journal Article|
|Title: The Journal of experimental medicine Volume: 135 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1972 Jan|
|Created Date: 1972-04-07 Completed Date: 1972-04-07 Revised Date: 2010-06-22|
Medline Journal Info:
|Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES|
|Languages: eng Pagination: 21-44 Citation Subset: AIM; IM|
|APA/MLA Format Download EndNote Download BibTex|
Cholesterol / metabolism*
Erythrocytes / metabolism
Esterases / metabolism
Esters / metabolism
Lipoproteins / metabolism
Lysosomes / metabolism
Macrophages / enzymology, metabolism*, physiology
Phospholipids / metabolism
|0/Carbon Isotopes; 0/Esters; 0/Linoleic Acids; 0/Lipoproteins; 0/Palmitic Acids; 0/Phospholipids; 10028-17-8/Tritium; 57-88-5/Cholesterol; EC 3.1.-/Esterases; EC 126.96.36.199/Acid Phosphatase|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Journal ID (nlm-ta): J Exp Med
Publisher: The Rockefeller University Press
Copyright © 1972 by The Rockefeller University Press
Received Day: 21 Month: 11 Year: 2009
Print publication date: Day: 1 Month: 1 Year: 1972
Volume: 135 Issue: 1
First Page: 21 Last Page: 44
PubMed Id: 4550608
|CHOLESTEROL METABOLISM IN THE MACROPHAGE : III. INGESTION AND INTRACELLULAR FATE OF CHOLESTEROL AND CHOLESTEROL ESTERS|
|Zanvil A. Cohn|
|From The Rockefeller University, New York 10021
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