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Cholesterol-linked pyrene excimer molecular beacon with enhanced cell permeability.
MedLine Citation:
PMID:  18776398     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Covelently labeled pyrene excimer molecular beacon (MB) with cholesterol moiety has been developed for enhanced the cellular delivery of MB.(1) Pyrene units were covalently attached into adenosine and incorporated to oligonucleotides at the complementary locations in opposite strands in the middle positions of hairpin stems. The system behaves as an effective MB that changes color from green to blue upon duplex formation. A cholesterol unit was also attached into a free terminus of one of these hairpins. The cholesterol-linked MBs enhanced the cellular delivery of the MBs and showed similar cell permeability to conventional transfection methods. These structurally simple cholesterol-based MB systems, which can be synthesized very efficiently, have good potential for opening up new and exciting opportunities in the field of in vivo biosensors.
Authors:
Hyun Seok Jeong; Young Jun Seo; Eun-Kyung Bang; Gil Tae Hwang; Jong Ha Jung; Sung Key Jang; Byeang Hyean Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids symposium series (2004)     Volume:  -     ISSN:  1746-8272     ISO Abbreviation:  Nucleic Acids Symp Ser (Oxf)     Publication Date:  2008  
Date Detail:
Created Date:  2008-09-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101259965     Medline TA:  Nucleic Acids Symp Ser (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  351-2     Citation Subset:  IM    
Affiliation:
Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, Korea.
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