Document Detail

Cholesterol-fed and casein-fed rabbit models of atherosclerosis. Part 2: Differing morphological severity of atherogenesis despite matched plasma cholesterol levels.
MedLine Citation:
PMID:  8274465     Owner:  NLM     Status:  MEDLINE    
One-month-old male New Zealand White rabbits were fed either a cholesterol-free casein diet (n = 10) or low-level cholesterol-supplemented chow (n = 10) for 24 weeks, during which total plasma cholesterol levels were matched. After perfusion fixation, aortic tissue samples were taken from six predetermined locations and embedded in epoxy resin for examination by light and electron microscopy. Frozen sections were also obtained for histochemical demonstration of collagen and elastin. Lesion morphology was classified in toluidine blue-stained, semithin epoxy sections as early fatty streaks (round foam cells with little intervening extracellular matrix); advanced fatty streaks (foam cells with extracellular lipid); fibrous plaques (spindle-shaped cells within extracellular matrix); or atheromatous lesions (presence of an atheromatous core). In representative specimens, electron microscopy showed that the ultrastructure of round foam cells was consistent with macrophage derivation, whereas most spindle-shaped cells were clearly smooth muscle cells. Fibrous plaques were more common in the distal than the proximal aorta. Lesions in the casein-fed animals were essentially equally distributed among the four morphological categories, whereas lesions in the cholesterol-fed rabbits were predominantly of the atheromatous type. Thus, cholesterol-fed rabbits had, in general, more advanced lesions than casein-fed rabbits with matched total plasma cholesterol levels. Moreover, the feeding of a low-level cholesterol diet (0.125% to 0.5% by weight) to rabbits for a relatively short time (6 months) led to the development of advanced lesions similar to those seen in humans.
S J Daley; K F Klemp; J R Guyton; K A Rogers
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Arteriosclerosis and thrombosis : a journal of vascular biology / American Heart Association     Volume:  14     ISSN:  1049-8834     ISO Abbreviation:  Arterioscler. Thromb.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-02-04     Completed Date:  1994-02-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9101388     Medline TA:  Arterioscler Thromb     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  105-41     Citation Subset:  IM    
Department of Anatomy, University of Western Ontario, London, Canada.
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MeSH Terms
Aorta / pathology
Arteriosclerosis / blood,  etiology*,  pathology
Caseins / administration & dosage*
Cholesterol / blood*
Cholesterol, Dietary / administration & dosage*
Collagen / analysis
Dietary Proteins / administration & dosage*
Disease Models, Animal*
Elastin / analysis
Microscopy, Electron
Reg. No./Substance:
0/Caseins; 0/Cholesterol, Dietary; 0/Dietary Proteins; 57-88-5/Cholesterol; 9007-34-5/Collagen; 9007-58-3/Elastin

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