Document Detail

Cholesterol esters regulate apoB48 secretion in CaCo2 cells.
MedLine Citation:
PMID:  11882317     Owner:  NLM     Status:  MEDLINE    
In this study, we investigated the effect of atorvastatin, an HMG-CoA reductase inhibitor and CL277082, an ACAT inhibitor, on apolipoprotein B48 synthesis, degradation and secretion in transformed human intestinal enterocytes (CaCo2 cells). Cells were incubated with atorvastatin or CL277082 in the absence or presence of sterol containing media and pulsed with [S35]-methionine and chased with unlabelled methionine. Concomitantly, the effect of atorvastatin and CL277082 on the relative amount of apoB48 protein in cells and media was also quantified by western blotting using an apoB antibody and enhanced chemiluminescence. Suppression of cholesterol synthesis with atorvastatin did not attenuate the production or secretion of apoB48 from CaCo2 cells under basal conditions. On the other hand, suppression of cholesterol biosynthesis with atorvastatin under stimulatory conditions accelerated the degradation of apoB48 in cells without affecting its synthesis or secretion. There was no effect of exogenous sterols on apoB48 secretion. Taken together, neither endogenous nor exogenous cholesterol appears to acutely modulate apoB48 secretion from intestinal cells. In contrast, inhibition of cholesterol esterification with ACAT inhibitor significantly attenuated apoB48 secretion under basal and stimulatory conditions by a mechanism which enhanced apoB48 degradation. Collectively, our results suggest that in CaCo2 cells, newly synthesized cholesterol ester may be an immediate regulator apoB48 secretion.
Sebely Pal; Emma Allister; Andrew Thomson; John C L Mamo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Atherosclerosis     Volume:  161     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-07     Completed Date:  2002-07-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  55-63     Citation Subset:  IM    
Department of Nutrition, Dietetics and Food Sciences, Curtin University of Technology, GPO Box U1987, Perth, WA, Australia.
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MeSH Terms
Apolipoprotein B-48
Apolipoproteins B / biosynthesis*,  secretion
Caco-2 Cells
Cholesterol / biosynthesis
Cholesterol Esters / biosynthesis*
Culture Media
Heptanoic Acids / pharmacology
Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology
Lipid Metabolism
Methionine / metabolism
Phenylurea Compounds / pharmacology
Pyrroles / pharmacology
Sterol O-Acyltransferase / antagonists & inhibitors
Reg. No./Substance:
0/Apolipoprotein B-48; 0/Apolipoproteins B; 0/Cholesterol Esters; 0/Culture Media; 0/Heptanoic Acids; 0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 0/Phenylurea Compounds; 0/Pyrroles; 110862-48-1/atorvastatin; 57-88-5/Cholesterol; 63-68-3/Methionine; 96224-26-9/CL 277082; EC O-Acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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