| Cholesterol esterification during differentiation of mouse erythroleukemia (Friend) cells. | |
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MedLine Citation:
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PMID: 22184540 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Cholesterol is an essential constituent of all mammalian cell membranes and its availability is therefore a prerequisite for cellular growth and other functions. Several lines of evidence are now indicating an association between alterations of cholesterol homeostasis and cell cycle progression. However, the role of cholesterol in cell differentiation is still largely unknown. To begin to address this issue, in this study we examined changes in cholesterol metabolism and in the mRNA levels of proteins involved in cholesterol import and esterification (multi-drug resistance, MDR-3) and acylCoA: cholesterol acyltransferase (ACAT) and cholesterol export (caveolin-1) in Friend virus-induced erythroleukemia cells (MELC), in the absence or in the presence of the chemical inducer of differentiation, hexamethylene bisacetamide (HMBA). FBS-stimulated growth of MELC was accompanied by an immediate elevation of cholesterol synthesis and cholesterol esterification, and by an increase in the levels of MDR-3 and ACAT mRNAs. A decrease in caveolin-1 expression was also observed. However, when MELC were treated with HMBA, the inhibition of DNA synthesis caused by HMBA treatment, was associated with a decrease in cholesterol esterification and in ACAT and MDR-3 mRNA levels and an increase in caveolin-1 mRNA. Detection of cytoplasmic neutral lipids by staining MELC with oil red O, a dye able to evidence CE but not FC, revealed that HMBA-treatment also reduced growth-stimulated accumulation of cholesterol ester to approximately the same extent as the ACAT inhibitor, SaH. Overall, these results indicate for the first time a role of cholesterol esterification and of some related genes in differentiation of erythroid cells. |
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Authors:
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Maria Franca Mulas; Antonella Mandas; Claudia Abete; Sandra Dessì; Alessandra Mocali; Francesco Paoletti |
Publication Detail:
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Type: Journal Article Date: 2011-10-19 |
Journal Detail:
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Title: Hematology reports Volume: 3 ISSN: 2038-8330 ISO Abbreviation: Hematol Rep Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-12-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101556723 Medline TA: Hematol Rep Country: Italy |
Other Details:
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Languages: eng Pagination: e19 Citation Subset: - |
Affiliation:
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Department of Internal Medicine, University of Cagliari, Cagliari; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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